| Zn(Ⅱ)-蛋白质络合物的极谱研究与应用 |
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| 引用本文： | 周春,罗登柏,韩英强,兰金贵.Zn(Ⅱ)-蛋白质络合物的极谱研究与应用[J].氨基酸和生物资源,2002,24(2):74-76. |
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| 作者姓名： | 周春 罗登柏 韩英强 兰金贵 |
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| 作者单位： | 中南民族学院化学系,湖北,武汉,430074 |
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| 摘 要： | 蛋白质的NaOH -盐酸胍溶液在 6 0℃恒温水浴中加热后与Zn(II)反应生成稳定的络合物 ,在 pH9.4的NaOH -NH4Cl缓冲介质中于单扫描示波极谱上产生一灵敏的极谱波 ,Ep在 - 0 .6 0V左右 (vs,SCE) ,其一阶导数波高Hp与蛋白质浓度成线性关系。相关系数R达 0 .998以上 ,检测限为 0 .1μg/mL。利用该波可以测定人血清样品中蛋白质的含量 ,结果与经典的考马斯亮蓝染色法相一致 ,方法具有简便、灵敏、取样量少等优点
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| 关 键 词： | 蛋白质 Zn(II)-蛋白质络合物 络合物吸附波 |
| 文章编号： | 1006-8376(2002)02-0074-03 |
| 修稿时间： | 2001年12月4日 |
Study and Application of Polarographic Adsorptive Wave of Protein-Zinc(Ⅱ) Complex |
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| ZHOU Chun,LUO Deng-bai,HAN Ying-qiang,LAN Jin-gui.Study and Application of Polarographic Adsorptive Wave of Protein-Zinc(Ⅱ) Complex[J].Amino Acids & Biotic Resources,2002,24(2):74-76. |
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| Authors: | ZHOU Chun LUO Deng-bai HAN Ying-qiang LAN Jin-gui |
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| Abstract: | The complex of Zinc(II) and proteins heated (60℃)for 20 minutes in presence of NaOH and guanidine hydrochloride produced a sensitive polarography in the buffer of NaOH-NH 4Cl, VC and CHCl 3 (pH9.4). Ep was at about-0.60V(vs SCE), and the wave height was linear to the concentration of proteins in the range of 0.2-35 μg/mL(R=0.998). The detection limit was 0.1ug/ml. The proposed method has been applied to determine HSA in blood samples with satisfactory results. |
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| Keywords: | proteins zinc(II)-proteins complex Complex Adsorption Wave |
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