| 来源于解淀粉芽胞杆菌的芸薹根肿菌抑菌活性物质的分离与鉴定 |
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| 引用本文： | 刘晓艳,张薇,闵勇,张亚妮,张志刚,江爱兵,杨自文.来源于解淀粉芽胞杆菌的芸薹根肿菌抑菌活性物质的分离与鉴定[J].氨基酸和生物资源,2014,36(1):39-43. |
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| 作者姓名： | 刘晓艳 张薇 闵勇 张亚妮 张志刚 江爱兵 杨自文 |
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| 作者单位： | 湖北省农业科学院湖北省生物农药工程研究中心,湖北武汉,430064;湖北生物科技职业学院园艺园林学院,湖北武汉,430070 |
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| 基金项目： | 国家科技支撑计划(项目编号：2011BAB06B004-02)国家863科技计划(项目编号：2011AA10A201) |
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| 摘 要： | 十字花科根肿病是由芸薹根肿菌引起的较为严重的世界性病害之一,至今还无有效控制该病害的生物农药。本研究通过盆栽实验,筛选到一株解淀粉芽胞杆菌HB_26,对根肿病原菌具有60%以上的抑制活性。通过高效液相质谱(LC_MS)对活性物质进行分离纯化,根据分子量和紫外吸收光谱初步鉴定活性物质为肽类,命名为BA30。抑真菌实验证明BA30在150μg·mL-1的浓度下,对小麦赤霉和灰霉病菌有70%的抑菌活性,对蚕豆锈病和水稻纹枯病菌有50%的抑菌活性,对番茄早疫病菌有30%的抑菌活性。
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| 关 键 词： | 十字花科根肿病 解淀粉芽胞杆菌 芸薹根肿菌 抗菌肽 分离与鉴定 |
Separation and Characterization of Bacillus amyloliquefaciens Active Substances Against Plasmodiophora brassicae |
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| Institution: | LIU Xiaoyan[1] ZHANG Wei[2] MIN Yong[1] ZHANG Yani[1] ZHANG Zhigang[1] JIANG Aibing[1] YANG Ziwen[1] |
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| Abstract: | Clubroot which is caused by Plasmodiophora brassicae,is a serious disease of brassica crops throughout the world. No any effective ways of controlling this pathogen is now available. B. amyloliquefaciens strain HB-26 with more than 60% inhibition activity against P. brassicae by Chinese cabbage pot experiment was studied. The active substance namely BA30 was separated and purified by HPLC,and it was identified to be a peptide derivative by mass spectrometry. In vitro inhibition test showed that BA30 had mutiple antifungal activity:inhibitory rate of 70% against Fusarium graminearum and Botrytis cinerea,50% against Uromyces fabae and Thanatephorus cucumeris,30% against Alternaria solani at the concentration of 150 μg·mL-1. Based on these results,we suggest that HB-26 or BA30 has potential application for the control of clubroot of brassica crops. |
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| Keywords: | clubroot Bacillus amyloliquefaciens Plasmodiophora brassicae antimicrobial peptide separation and characterization |
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