| 利用反相高效液相色谱测定香椿叶中槲皮素含量 |
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| 引用本文： | 李颖,王昌禄,刘常金,夏廉法,陈志强,陈勉华,江慎华.利用反相高效液相色谱测定香椿叶中槲皮素含量[J].氨基酸和生物资源,2006,28(4):74-76. |
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| 作者姓名： | 李颖 王昌禄 刘常金 夏廉法 陈志强 陈勉华 江慎华 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津市食品营养与安全重点实验室,天津,300457;河南省农业科学院农产品加工研究所,河南,郑州,450002 |
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| 摘 要： | 用乙醇对香椿叶粉末进行提取,树脂柱浓缩,真空冷冻干燥,甲醇溶解,制备香椿黄酮。用7.6 mol.L-1盐酸85℃水浴回流2 h进行水解,以反相ODS柱甲醇-水(体积比为50:50,磷酸调pH至2.53)为流动相,在波长368 nm处对香椿叶中的槲皮素进行分离、测定。结果表明:香椿叶中含槲皮素平均质量分数为1.28%,平均加样回收率为98.9%,RSD为1.75%,反相高效液相色谱法测定香椿叶中槲皮素含量操作简便易行、结果准确可靠。
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| 关 键 词： | 香椿叶 槲皮素 反相高效液相色谱 |
| 文章编号： | 1006-8376(2006)-04-0074-03 |
| 收稿时间： | 08 10 2006 12:00AM |
| 修稿时间： | 2006年8月10日 |
Determination of Quercetin in Leaves of Toona sinensis by Reversed- Phase High Performance Liquid Chromatography |
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| LI Ying,WANG Chang-lu,LIU Chang-jin,XIA Lian-fa,CHEN Zhi-qiang,CHEN Mian-hua,JIANG Shen-hua.Determination of Quercetin in Leaves of Toona sinensis by Reversed- Phase High Performance Liquid Chromatography[J].Amino Acids & Biotic Resources,2006,28(4):74-76. |
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| Authors: | LI Ying WANG Chang-lu LIU Chang-jin XIA Lian-fa CHEN Zhi-qiang CHEN Mian-hua JIANG Shen-hua |
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| Institution: | LI Ying~1,WANG Chang-lu~ |
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| Abstract: | A RP-HPLC method for the determination of quercetin in leaves of Toona sinensis was developed.Samples were mixed with HCl of 25%,which were heated at 85℃ for 2 hours.Quercetin was separated completely on an ODS column(4.6mm×250mm),using a mixture of methanol and water(50∶50,phosphoric acid made the pH 2.53) as mobile phase,with UV detection at 368nm.The results have shown that the content of quercetin was up to 1.28%,the recovery rate was 98.9% and RSD was 1.75%.The method is quick and simple with good accuracy and can be used for the quality control. |
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| Keywords: | leaves of Toona sinensis quercetin reversed-phase high performance liquid chromatography |
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