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黑曲霉产木聚糖酶发酵条件的研究
引用本文:吴小刚,黄东平,曾莹.黑曲霉产木聚糖酶发酵条件的研究[J].氨基酸和生物资源,2007,29(2):26-29.
作者姓名:吴小刚  黄东平  曾莹
作者单位:1. 湖北工业大学,生物工程学院,湖北,武汉,430068;2. 武汉华珍药业有限公司,湖北,武汉,430051
基金项目:湖北省科技厅重点攻关项目2004AA104B03
摘    要:将经过诱变选育高产木聚糖酶并具有Nystatin抗性的黑曲霉,分别在不同条件下进行固体发酵培养,探讨最佳产酶条件。结果显示:在以质量分数1%木糖为附加碳源,以质量分数2%NH4NO3为氮源,无机盐为质量分数1%NaCl,加水比例为1:1.3,接种量为1.5%,装料量为5g/300m l三角瓶,培养温度为30℃,培养周期为72 h的培养条件下,菌株产木聚糖酶活力提高至7285.4 IU.g-1。比出发菌株提高了20%。酶活力测定采用3,5-二硝基水杨酸(DNS)法。

关 键 词:木聚糖酶  发酵条件  酶活力
文章编号:1006-8376(2007)02-0026-04
修稿时间:2006-12-20

Study on Fermentation Conditions of Aspergillus niger Producing Xylanase
WU Xiao-gang,HUANG Tong-ping,ZENG ying.Study on Fermentation Conditions of Aspergillus niger Producing Xylanase[J].Amino Acids & Biotic Resources,2007,29(2):26-29.
Authors:WU Xiao-gang  HUANG Tong-ping  ZENG ying
Institution:1. Bioengineering Department, Hubei Technology University, Wuhan 430068 ; 2. Wuhan Huazheng Medicine Limited Company, Wuhan 430051, China
Abstract:The optimal fermentation conditions for a Aspergillus niger mutant producing xylanare and resisting to nystatin were studied.The results were as follows: the content of xylose was 1%;the content of NH4NO3 was 2%;the content of inorganic salt was Nacl 1%;the ratio of water to medium was 1:1.3;the inoculum was 1.5%;the forced filling rate was 20g to 300ml bottle;the temperature is 30℃;the fermentation time is 72 hours.Under those conditions,the xylanase activity was up to 7285.4IU·g-1 and 20% higher than the original.
Keywords:xylanase  fermentation condition  enzymatic activity
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