| 谷氨酰胺高效酶法转化条件研究 |
| |
| 引用本文： | 周丛,李宗军.谷氨酰胺高效酶法转化条件研究[J].氨基酸和生物资源,2009,31(2):46-49. |
| |
| 作者姓名： | 周丛 李宗军 |
| |
| 作者单位： | 国家农副产品质量监督检验中心;湖南农业大学食品科技学院,中国湖南,长沙,410007 |
| |
| 摘 要： | 谷氨酰胺合成酶是生物体氮代谢的中心酶之一,在消耗ATP的情况下,谷氨酰胺合成酶催化由谷氨酸和NH4+向谷氨酰胺的转化,Toch ikura提出了将酵母发酵与纯化酶结合生产谷氨酰胺(G ln)的方法,本实验通过建立酶法合成L-G ln与酵母酒精发酵的能量偶联体系,研究了在此偶联体系中各因素对谷氨酰胺酶转化效率的影响,为工业上利用酶法生产G ln提供理论依据。
|
| 关 键 词： | L-谷氨酰胺 生物酶法 转化条件 |
The study of Efficient Enzymatic Conversion of Glutamine |
| |
| ZHOU Cong,LI Zong-jun.The study of Efficient Enzymatic Conversion of Glutamine[J].Amino Acids & Biotic Resources,2009,31(2):46-49. |
| |
| Authors: | ZHOU Cong LI Zong-jun |
| |
| Institution: | ZHOU Cong1,LI Zong-jun2(1.National Center for Quality Supervision , Inspection of Agricultural , Sideline Products,2.Hunan Agricultural University,Changsha 410007,China) |
| |
| Abstract: | Glutamine synthetase is very important enzyme for human.By ATP consumption,glutamine synthetase catalyzes glutamic acid and NH4+ to conversion of glutamine,Tochikura have made the yeast fermentation combined with the net production of glutamine.We established energy coupling system of enzymatic synthesis of L-Gln and the yeast fermentation of alcohol,and different factors influenced the glutamine conversion were studied. |
| |
| Keywords: | L-Gln enzymatic method conversion conditions |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
