椰心叶甲、椰子织蛾肠道菌群多样性分析

目的] 椰心叶甲、椰子织蛾同属棕榈科食叶性入侵害虫，两者同时取食棕榈科植物却无明显竞争作用，本文通过分子生物学手段对两者的肠道菌群组成、肠道代谢通路进行比对，以此探讨两者食性之间相关性。方法] 以16S rRNA菌群分析等预测手段对两者肠道微生物群落、代谢通路等进行差异分析。结果] 椰心叶甲体内变形菌门群落丰度显著高于椰子织蛾，椰子织蛾体内蓝菌门、放线菌门、酸杆菌门、疣微菌门等菌群丰度显著高于椰心叶甲；椰心叶甲体内膦酸酯和亚膦酸盐代谢、N-聚糖合成等肠道代谢通路显著强于椰子织蛾，而椰子织蛾肠道中酮体的合成和降解、维生素B6代谢、核黄素代谢、黄酮和黄酮醇生物合成等代谢通路显著强于椰心叶甲。结论] 椰子织蛾体内相对较高的酸杆菌门丰度可能导致了其偏好取食纤维素高的椰子老叶，而椰心叶甲则相反。同时，椰子织蛾肠道内显著较高的维生素B含量在其族群发展过程中起到了主要作用。两者细菌丰度以及代谢通路强度的差异可能影响了两者的食性。

Diversity analysis of gut microbes in Brontispa longissima and Opisina arenosella

Aim] Brontispa longissima and Opisina arenosella belong to the leafk-eating invasive pests of the Palmaceae, but they do not compete with each other when they eat plants at the same time. This article uses molecular biology methods to compare the intestinal flora composition and intestinal metabolic pathways of B. longissima and O. arenosella, in order to explore the correlation between the diets of them, and provide a basis for the comprehensive prevention and control of them.Method] The 16S rRNA flora analysis and other prediction methods were used to analyze the intestinal microbial community and metabolic pathways of the two species.Result] The results showed that the abundance of the Saccharibacteria community in B. longissima was significantly higher than that of O. arenosella. The abundance of Cyanobacteria, Actinobacteria, Acidobacteria, Verrucomicrobia, etc. in O. arenosella is significantly higher than that of B. longissima. The degree of intestinal metabolic pathways such as phosphonate and phosphinate metabolism, N-glycan synthesis in B. longissima is significantly stronger than that of O. arenosella, and the synthesis and degradation of ketone bodies, vitamin B6 metabolic pathways such as metabolism, riboflavin metabolism, flavonoids and flavonol biosynthesis in the intestine of O. arenosella are significantly stronger than those of B. longissima.Conclusion] The relatively high abundance of Acidobacter spp. in O. arenosella may lead to its preference to eat old coconut leaves with high fiber content, but B. longissima is the opposite; At the same time, the significantly stronger metabolic strength of vitamin B6 in O. arenosella plays an important role in the construction of its spleen, and promotes the establishment of its ethnic group. The differences in bacterial abundance and the strength of metabolic pathways between the two species may affect their feeding habits.