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从日本进境邮寄物中截获伤残短体线虫
引用本文:闫世艳,陈艳,边勇,高文娜,简恒,李倞平,张继军.从日本进境邮寄物中截获伤残短体线虫[J].生物安全学报,2016,25(4):291-294.
作者姓名:闫世艳  陈艳  边勇  高文娜  简恒  李倞平  张继军
作者单位:北京农学院;中国农业大学;北京出入境检验检疫局
基金项目:北京市教委2015年度科研计划项目(KM201510020004);国家质检总局科技计划项目(2015IK023)
摘    要:【背景】2015年北京出入境检验检疫技术中心植物实验室从日本邮寄进境的鸡爪槭中检出一种短体线虫。【方法】通过采用形态学鉴定、rDNA序列测定及系统进化分析,对该线虫进行鉴定。【结果】将该线虫的形态特征和测计值与已知种群比较,与Pratylenchus vulnus最为接近;系统进化分析表明,其r DNA-ITS区序列特征与P.vulnus序列相似度为99%;系统发育树表明,其与P.vulnus聚为一组,证实该线虫为伤残短体线虫P.vulnus。【结论与意义】伤残短体线虫是一种重要的植物寄生线虫,我国进出境检疫部门曾多次从入境货物中检出该线虫,但从入境邮寄物中检中尚属首次。

关 键 词:邮寄物检疫  伤残短体线虫  鸡爪槭  rDNA-ITS  系统发育  鉴定
收稿时间:2016/3/1 0:00:00
修稿时间:2016/5/6 0:00:00

Root-lesion nematode, Pratylenchus vulnus, on imported mails from Japan
Shi-yan YAN,Yan CHEN,Yong BIAN,Wen-na GAO,Heng JIAN,Jing-ping LI and Ji-jun ZHANG.Root-lesion nematode, Pratylenchus vulnus, on imported mails from Japan[J].Journal of Biosafety,2016,25(4):291-294.
Authors:Shi-yan YAN  Yan CHEN  Yong BIAN  Wen-na GAO  Heng JIAN  Jing-ping LI and Ji-jun ZHANG
Institution:Beijing University of Agriculture, Beijing 102206, China;China Agriculture University, Beijing 100193, China,Beijing University of Agriculture, Beijing 102206, China,Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100226, China,Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100226, China,China Agriculture University, Beijing 100193, China,Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100226, China and Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100226, China
Abstract:Background] In 2015,one Pratylenchus population was collected in the roots and growing media of Acer palmatum imported from Japan by plant quarantine lab of Beijing entry-exit inspection and quarantine bureau, China.Method] In the study the population was identified by morphological measurements, rDNA sequence and phylogenetic analysis.Result] Comparing the morphological characteristics and measurements with known species, the nematode is phylogenetically close to Pratylenchus vulnus, accroding to the sequence of rDNA-ITS region with 99%. The results confirmed that this population was P. vulnus.Conclusion and significance] P. vulnus is an important plant parasitic nematodes that has been intercepted from the entry of goods many times by our entry-exit inspection and quarantine departments, but it is the first time in the mail quarantine.
Keywords:mail quarantine  Pratylenchus vulnus  Acer palmatum  rDNA-ITS  phylogenetic analysis  identification
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