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不同辣椒品种抗(耐)西花蓟马的初步筛选及苗期防御酶的变化
引用本文:符伟,朱春晖,刘勇,张德咏,谭新球.不同辣椒品种抗(耐)西花蓟马的初步筛选及苗期防御酶的变化[J].生物安全学报,2013,22(1):28-32.
作者姓名:符伟  朱春晖  刘勇  张德咏  谭新球
作者单位:湖南省农业科学院植物保护研究所,园艺作物病虫害综合治理湖南省重点实验室;湖南农业大学农药研究所
基金项目:国家公益性行业(农业)科研专项(201103026)
摘    要:【背景】西花蓟马自2003年传入我国以来,呈扩张趋势。辣椒上西花蓟马以化学防治为主,国内尚无抗性资源保护、抗(耐)虫性资源筛选等相关研究。【方法】以20个辣椒品种和西花蓟马为材料,采用幼苗接虫法,根据危害症状分级,计算为害指数并作为抗(耐)评价指标,然后测定不同抗(耐)材料苗期受西花蓟马危害后防御酶活性的变化,探讨其与抗性的关系。【结果】在20个供试辣椒品种中,湘研13号和博辣4号对西花蓟马的抗性较强,其他品种抗性较差,兴蔬绿剑抗性最差;不同辣椒品种抗(耐)虫性与过氧化物酶变化呈正相关,但与过氧化氢酶相关性不显著。【结论与意义】本研究建立了辣椒抗(耐)西花蓟马的筛选评价体系,为挖掘抗西花蓟马的种质资源和西花蓟马的有效防控提供了依据。

关 键 词:西花蓟马  辣椒品种  抗(耐)虫性  植物防御酶

Screening on different Capscium annuum cultivars resistant/tolerant to the western flower thrips (Frankliniella occidentalis) and variation on their defense enzymes at seedlings stage
Wei FU,Chun-hui ZHU,Yong LIU,De-yong ZHANG and Xin-qiu TAN.Screening on different Capscium annuum cultivars resistant/tolerant to the western flower thrips (Frankliniella occidentalis) and variation on their defense enzymes at seedlings stage[J].Journal of Biosafety,2013,22(1):28-32.
Authors:Wei FU  Chun-hui ZHU  Yong LIU  De-yong ZHANG and Xin-qiu TAN
Institution:1* 1Key Laboratory of Integrate Diseases and Insects Pests Management in Horticultural Crops in Hunan, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China; 2Pesticides Institute, Hunan Agricultural University, Changsha, Hunan 410128, China
Abstract:【Background】The western flower thrips (Frankliniella occidentalis) was introduced into China in 2003, and its occurrence and damage is increaseing. Chemical control is currently widely used but unsustainable. This work is aimed at screening and identification of potentially resistant germplasm resources. 【Method】Juvenile seedlings of different Capsicum annuum cultivars were infested with 1st nympha instar F.occidentalis in glasshouse, and the index of damage was calculated by revised damaged symptoms and graded standard. The correlation between the activity of plant defense enzymes and resistance levels was analyzed by the activity variation of calatase (CAT) and peroxidase (POD). 【Result】Two out of 20 tested cultivars, Xiangyan No.13 and Bola No.4 demonstrated high levels of resistance/tolerance, while the others had middle level of resistance/tolerance or were susceptible. Cv. Xingshu Lüjian the most susceptible cultivar. A positive relationship was observed between POD activity and resistance level on selected cultivars, but CAT did not seem to be related to resistence. 【Conclusion and significance】In the study, the method of evaluation and screening resistance/tolerance to western flower thrips (F.occidentalis) on different C.annuum cultivars at the juvenile seedlings stage in glasshouse was developed, which are proof of discovering the resistant plant germplasm resources and further effective control to western flower thrips.
Keywords:western flower thrip  Capscium annuum cultivar  pest resistance/tolerance  plant defense enzyme
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