黄瓜资源对黄瓜绿斑驳病毒广东分离物的抗性水平鉴定

【背景】黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)是严重威胁葫芦科作物生产的毁灭性病原之一,该病毒已入侵我国十多个省份,危害西瓜、黄瓜等作物并造成严重的经济损失。早在2009年广东即发现CGMMV为害西瓜和黄瓜,但黄瓜等葫芦科作物对其抗性情况尚不清楚。【方法】采用人工机械摩擦接种方法,测定了14份黄瓜种质资源对CGMMV广东分离物的抗性水平。【结果】从广东葫芦病样中分离获得CGMMV,该病毒分离物MP基因序列与国内报道的各分离物同源率均在99%以上;14份黄瓜种质资源对该病毒分离物均表现为感病。【结论与意义】广东主要黄瓜资源对CGMMV均表现为感病,这为我省防控该病毒病提供了科学依据,也为黄瓜抗病育种提供了指导。

Identification of cucumber germplasm resistance to Cucumber green mottle virus Guangdong isolate

Background] Cucumber green mottle mosaic virus (CGMMV) is one of the most devastating pathogens of cucurbit crops. The virus has invaded more than ten provinces of China and infected crops such as watermelon and cucumber, causing serious economic losses. CGMMV infected watermelon and cucumber in Guangdong Province in 2009, but the resistance of cucumber and other cucurbits was unknomn.Method] The resistance of 14 cucumber germplasms to a CGMMV isolate was tested using artificial mechanical inoculation and nucleotide sequencing.Result] The MP nucleotide sequence of CGMMV of Guangdong isolate from the diseased cucurbit samples was 99% similar to other isolates of CGMMV collected in China. All of 14 cucumber germplasms were susceptible to the isolate of CGMMV.Conclusion and significance] The main cucumber germplasms from Guangdong are not resistant to CGMMV. This result provides a scientific basis for control of CGMMV, and some guidance for cucumber breeding to resist CGMMV in Guangdong.