烟粉虱携带番茄黄化曲叶病毒检测及其传入山东省的考证

【背景】番茄黄化曲叶病毒（TYLCV）是由媒介昆虫烟粉虱传播的一种双生病毒，对蔬菜及烟草等经济作物造成严重危害。前人资料表明，该病毒于2006年传人我国南方地区，2007年传人山东省，2008年后在山东各地逐渐蔓延扩散。【方法】为了考证TYLcV传人山东省的时间，本研究利用mtCOI基因对于2005和2006年7—8月份在山东省不同地区作物上共采集的15份烟粉虱样品进行了生物型鉴定，并进一步检测了烟粉虱携带TYLCV情况，同时对PCR扩增产物进行了测序分析。【结果】2005年的4份样品烟粉虱生物型均为B型，均不携带TYLCV。2006年的11份烟粉虱样品为B型与Q型混合样品，其中，2份烟粉虱样品检测到TYLCV，进一步证实该病毒为TYLCV。【结论与意义】本研究首次证实了TYLCV早在2006年就已经传入山东省。研究结果不仅对于防控该病毒具有重要指导意义，而且对于其入侵生物学研究也具有重要参考价值。

Tomato yellow leaf curl virus has been introduced in Shandong Province since 2006 based on the detection of the virus within vector whitefly, Bemisia tabaci (Gennadius)

Background ] Tomato yellow leaf curl virus （TYLCV） is one of geminiviruses, which is transmitted by the sweet potato whitefly, Bemisia tabaci. The virus can cause serious damage to economic crops, such as vegetables and tobaccoes. Previous data indicated that the virus was introduced into the southern China in 2006 and then into Shandong in 2007. The virus has been gradual ly spreading throughout Shandong Province since 2008. Method ] In order to determine the dispersal time of TYLCV in Shandong Province, we surveyed 15 B. tabaci samples collected from different counties in the province from July to August in 2005 and 2006, respectively. Result] The results showed that the 4 B. tabaci samples in 2005 were biotype B and all of them were nonviruliferrous. The 11 B. tabaci samples in 2006 were mixed with biotypes B and Q. TYLCV was only detected within 2 of 11 samples in 2006. Mo lecular analysis confirmed that the virus was TYLCV. Conclusion and significance] The study proved for the first time that it was at least in 2006 that the TYLCV was introduced into Shandong Province. The results have important guidance not only for the preven tion and control of this virus, but also have important reference value for the invasion biology of this virus.