脱氧核酶抑制Akt1基因对MCF-7乳腺癌细胞生长的影响 |
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引用本文: | 张敏,赵航,付强,施维.脱氧核酶抑制Akt1基因对MCF-7乳腺癌细胞生长的影响[J].国外医学:分子生物学分册,2011(4):323-326. |
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作者姓名: | 张敏 赵航 付强 施维 |
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作者单位: | [1]吉林大学分子酶学工程教育部重点实验室,长春市130012 [2]北华大学,吉林省吉林市132001 |
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基金项目: | 吉林省科技厅项目(No.20080932) |
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摘 要: | 目的应用脱氧核酶抑制Akt1的表达,观察MCF-7乳腺癌细胞生长及凋亡情况。方法采用噻唑蓝比色法(MTT)检测脱氧核酶抑制MCF-7乳腺癌细胞增殖作用;DAPI染色法分析细胞凋亡形态学的变化;流式细胞术检测脱氧核酶对MCF-7乳腺癌细胞凋亡的影响;运用蛋白免疫印迹检测分析Akt1、pro—caspase-3、pro-caspase-9的变化。结果Aktl脱氧核酶对MCF-7乳腺癌细胞在体外的生长具有抑制作用;DRz1组的细胞早期凋亡率显著高于未处理组;荧光显微镜下可见典型的凋亡形态学变化;脱氧核酶作用后,免疫印迹检测Aktl蛋白表达降低,pro—caspase-3、9均被活化。结论AktlDRzl能有效下调MCF-7乳腺癌细胞Akt1的蛋白表达水平,抑制MCF-7细胞的生长,且凋亡途径可能依赖于caspase-3、9的相关的线粒体凋亡途径。
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关 键 词: | Akt1基因 脱氧核酶 细胞凋亡 |
Inhibitory Effect of Aktl Deoxyribozymes on MCF-7 Cell Growth |
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Authors: | ZHANG Min ZHAO Hang FU Qiang SHI Wei |
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Institution: | 1 Key Laboratory for Molecular Enzymology & Engineering of the Ministry of Education, Jilin University, Changchun, 130012, China 2Beihua University, Jilin, Jilin Province, 132001, China) |
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Abstract: | Objective To investigate the effect of deoxyribozymes (DRz) that inhibits Aktl on apoptosis of MCF-7 cells. Methods Inhibitive effect of DRz on MCF-7 cell growth was determined with MTF. Apoptosis induced by DRz was detected by Annexin V-FITC/PI double stai- ning. Apoptotic morphology was shown by DAPI staining. Aktl expression was analyzed by Real time- PCR and Western blot. Results DRz significantly inhibited proliferation with decrease of Aktl expression and induced apoptosis of MCF-7 cells with activation of pro-caspase-3 and 9, as determined by western blot and quantitative analysis. Conclusion DRz inhibits Akt expression and thus induces apoptosis of MCF-7 cells. |
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Keywords: | Akt1 DNAzyme cell apoptosis |
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