| 脱氧核酶抑制Akt1基因对MCF-7乳腺癌细胞生长的影响 |
| |
| 引用本文： | 张敏,赵航,付强,施维.脱氧核酶抑制Akt1基因对MCF-7乳腺癌细胞生长的影响[J].国外医学：分子生物学分册,2011(4):323-326. |
| |
| 作者姓名： | 张敏 赵航 付强 施维 |
| |
| 作者单位： | [1]吉林大学分子酶学工程教育部重点实验室,长春市130012 [2]北华大学,吉林省吉林市132001 |
| |
| 基金项目： | 吉林省科技厅项目（No.20080932） |
| |
| 摘 要： | 目的应用脱氧核酶抑制Akt1的表达，观察MCF-7乳腺癌细胞生长及凋亡情况。方法采用噻唑蓝比色法（MTT）检测脱氧核酶抑制MCF-7乳腺癌细胞增殖作用；DAPI染色法分析细胞凋亡形态学的变化；流式细胞术检测脱氧核酶对MCF-7乳腺癌细胞凋亡的影响；运用蛋白免疫印迹检测分析Akt1、pro—caspase-3、pro-caspase-9的变化。结果Aktl脱氧核酶对MCF-7乳腺癌细胞在体外的生长具有抑制作用；DRz1组的细胞早期凋亡率显著高于未处理组；荧光显微镜下可见典型的凋亡形态学变化；脱氧核酶作用后，免疫印迹检测Aktl蛋白表达降低，pro—caspase-3、9均被活化。结论AktlDRzl能有效下调MCF-7乳腺癌细胞Akt1的蛋白表达水平，抑制MCF-7细胞的生长，且凋亡途径可能依赖于caspase-3、9的相关的线粒体凋亡途径。
|
| 关 键 词： | Akt1基因 脱氧核酶 细胞凋亡 |
Inhibitory Effect of Aktl Deoxyribozymes on MCF-7 Cell Growth |
| |
| Authors: | ZHANG Min ZHAO Hang FU Qiang SHI Wei |
| |
| Institution: | 1 Key Laboratory for Molecular Enzymology ＆ Engineering of the Ministry of Education, Jilin University, Changchun, 130012, China 2Beihua University, Jilin, Jilin Province, 132001, China) |
| |
| Abstract: | Objective To investigate the effect of deoxyribozymes （DRz） that inhibits Aktl on apoptosis of MCF-7 cells. Methods Inhibitive effect of DRz on MCF-7 cell growth was determined with MTF. Apoptosis induced by DRz was detected by Annexin V-FITC/PI double stai- ning. Apoptotic morphology was shown by DAPI staining. Aktl expression was analyzed by Real time- PCR and Western blot. Results DRz significantly inhibited proliferation with decrease of Aktl expression and induced apoptosis of MCF-7 cells with activation of pro-caspase-3 and 9, as determined by western blot and quantitative analysis. Conclusion DRz inhibits Akt expression and thus induces apoptosis of MCF-7 cells. |
| |
| Keywords: | Akt1 DNAzyme cell apoptosis |
| 本文献已被 维普 等数据库收录！ |
