| HeLa细胞中缝隙连接蛋白Cx26/Cx32的表达及其功能调控 |
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| 引用本文： | 童旭辉,董淑英,陶亮.HeLa细胞中缝隙连接蛋白Cx26/Cx32的表达及其功能调控[J].国外医学：分子生物学分册,2011(3):245-247. |
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| 作者姓名： | 童旭辉 董淑英 陶亮 |
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| 作者单位： | [1]蚌埠医学院药学系药理教研室安徽省生化药物工程技术研究中心,安徽省蚌埠市233000 [2]中山大学中山医学院药理教研室,广州市510080 |
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| 基金项目： | 国家自然科学基金（No.81001457）,安徽省教育厅省级优秀青年人才基金（No.2010SQRL121ZD）,安徽省教育厅高校省级科学研究项目（No.kj2010B117） |
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| 摘 要： | 目的 在HeLa宫颈癌细胞中研究不同浓度的多西环素对缝隙连接蛋白Cx26/Cx32表达及由其形成的缝隙连接通讯功能的影响.方法 采用Western印迹检测HeLa细胞中Cx26/Cx32的蛋白表达;荧光示踪实验用于检测HeLa细胞中由Cx26/Cx32形成的缝隙连接通讯功能.结果 Western印迹结果显示多西环素在0.01～1 μg/ml的范围内,随着剂量的增加,Cx26/Cx32蛋白表达水平增加;荧光示踪实验结果显示HeLa细胞之间的荧光传递随着多西环素增加也相应增强.结论 采用加入不同浓度多西环素的方法,可制备缝隙连接通讯功能强弱不同的细胞模型.
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| 关 键 词： | 多西环素 Cx26/Cx32 缝隙连接 |
Expression and Functional Regulation of Gap Junction Proteins Cx26/Cx32 in HeLa Cells |
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| Authors: | TONG Xuhui DONG Shuying TAO Liang |
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| Institution: | 1Department of Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals, Bengbu, Anhui, 233000, China 2Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China) |
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| Abstract: | Objective To determine the effect of doxycycline in different concentrations on the expression of Cx26/Cx32 proteins and their function in gap junction intercellular communication in HeLa cervical cancer cells. Methods Western blotting was used to detect Cx26/Cx32 protein in HeLa cells. Fluorescence trace was used to assay the gap junction intercellular communication media- ted by Cx26/Cx32 in HeLa cells. Results The expression of Cx26/Cx32 was increased with the doxyeycline concentration from O. O1 to 11xg/ml, as determined by western blotting. The fluores- cence trace was enhanced with the increased concentration of doxycycline. Conclusion The cellular models of different gap junction intercellular communication were preparable by treatment with different concentrations of doxycyetine. |
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| Keywords: | doxycycline Cx26/Cx32 gap junction |
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