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DNA-free RNA preparations from mycobacteria |
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| Authors: | Joachim?Stephan Johannes?G?Bail Fritz?Titgemeyer |
| Institution: | (1) Lehrstuhl fr Mikrobiologie, Friedrich-Alexander-Universitt Erlangen-Nrnberg, Staudtstr. 5, D-91058, Erlangen, Germany;(2) Department of Microbiology,University of Alabama at Birmingham, 609 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294, USA |
| Abstract: | BackgroundTo understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. |
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