| L—赖氨酸高产菌株选育的研究 |
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| 引用本文： | 齐秀兰,阎浩林.L—赖氨酸高产菌株选育的研究[J].微生物学杂志,1997,17(2):12-16. |
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| 作者姓名： | 齐秀兰 阎浩林 |
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| 作者单位： | 沈阳药科大学微生物教研室!110015 |
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| 摘 要： | L-赖氨酸产生菌钝齿棒杆菌（Corynebacteriumcrenatum）N30－25菌株经紫外线诱变处理，分别在含有不同浓度的七叶苷的培养基上进行筛选，经摇瓶多次复筛获得了3株高产变异菌株。对这3株菌在相同发酵条件下进行发酵生产L－赖氨酸，与出发菌株比较，产量提高了22－31％，经过3次传代，产生L－赖氨酸能力仍很稳定。
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| 关 键 词： | 钝齿棒杆菌 L－赖氨酸 七叶苷 |
A STUDY ON THE SELECTIOM OF HIGH-YIELD LYSINE PRODUCTING STRAINS |
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| Qi Xiulan,et al..A STUDY ON THE SELECTIOM OF HIGH-YIELD LYSINE PRODUCTING STRAINS[J].Journal of Microbiology,1997,17(2):12-16. |
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| Authors: | Qi Xiulan |
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| Institution: | Qi Xiulan, et al. |
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| Abstract: | After treated by ultraviolet, lysine producing strain Corynebacte-rium crenatum N30-25 was cultivated on the solid media contained different concentration of esculin. Three highyield lysine producing mutants were obtained with repeat screening in shake flasks. The yield of lysine of the three mutants were increased by 22-31% as compared with that of initial strain under the same condition. The ability of producing lysine of the mutants have been studied after three regene-ration. |
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| Keywords: | Corynebacterium crenatum L-lysine esculin |
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