杀线虫天然产物Chaetoglobosin A合成的表观遗传调控证据

通过高效液相色谱(HPLC)、高分辨率质谱(HRMS)和~H和~(13)C核磁共振(NMR)证明1株球毛壳菌Chaetomiumglobosum NKl02产生的杀根结线虫(Meloidogyne spp.)成分为球毛壳甲素(Chaetoglobosin A)。C.globosum NK102能够利用微晶纤维素、杨树叶和木屑合成ChA。在12 d发酵液中,3种培养基获得的ChA产量由高到低的顺序为PDA、杨树叶、微晶纤维素和木屑,产量分别为26.49、9.38、4.47和0.47 mg/L。通过在微晶纤维素培养基中添加表观遗传沉默抑制药物表明:100μmol/L 5′-AZA的添加使得ChA的产量提高至原来的3.6倍,100μmol/L SAHA将ChA的产量提高到原来的2.4倍,DMSO使得ChA的产量提高到原来的1.7倍。这个结果预示ChA的合成的某些基因受到表观遗传调控。

Evidence for Epigenetic Regulation of Nematicide Natural Product Chaetoglobosin A Biosynthesis in Chaetomium globosum NK102

A chemical secreted by Chaetomium globosum NK102 had nematicidal activity against Meloidogyne incognita.This chemical was characterized to be chaetoglobosin A （ChA） by HPLC,high resolution mass spectrometry （HRMS） and 1H and 13C NMR.C.globosum NK102 could utilize microcrystalline cellulose,leaves,and wood filings of poplar to synthesize ChA.The yield of ChA in 12 d culture medium was 9.38 mg/L with poplar leaves,4.47 mg/L with microcrystalline cellulose,and 0.47 mg/L with wood filings.The highest yield obtained was 26.49 mg/L with PDA.By adding epigenetic silent inhibition drug into microcrystalline cellulose medium,indicated that addition of 100 μmol/L 5＇-AZA made the yield of ChA increased 3.6 times of the origin,100 μmol/L 2.4 times of the origin,and 200 μL DMSO 1.7 times of the origin.The results predicted that the synthesis of ChA was epigenetically regulated by some genes.