貂源乳酸菌增菌培养基优化

为实现貂源乳酸菌制剂中试生产,本实验选择多种碳、氮源,通过对不同碳、氮源进行优化,明确貂源植物乳杆菌MDL1118增菌培养基配方为蔗糖5%、玉米浆干粉7.5%、番茄汁10%、无机盐0.5%、tween-800.25%。在此培养基中,37℃搅拌发酵7 h,活菌数可达到3.18×109cfu/m L,与以葡萄糖为主要碳源培养基相比,增菌效果明显。

Optimization of Proliferation Medium for Mink Source Lactobacilli

Multiple carbon and nitrogen sources were selected to optimize the proliferation medium for mink source lactic acid bacteria preparation for realization of pilot production. The results shown that the proliferation medium formula for plant lactobacillus MDL1118 of mink source was as follow： sucrose 5%,corn syrup powder 7. 5%,tomato juice 10%,mineral salts 0. 5%,and tween-80 0. 25%. The living cell was as much as 3. 18 × 109 cfu / m L with this medium and stirring culture for 7 h at 37 ℃. As compared with medium with glucose as main carbon source the proliferation effect was significant.