4种珍稀食用菌水提物的抗氧化活性研究

用DPPH自由基清除法、羟基自由基清除法和超氧阴离子自由基清除法对4种珍稀食用菌灵芝、云芝、茶树菇、松茸的水提物进行抗氧化活性评价，为更好评价其抗氧化活性，以维生素C作为阳性对照。实验结果显示：4种食用菌水提物具有不同程度的抗氧化活性。云芝对DPPH自由基的清除能力最强，其IC₅₀值为1.46 mg/mL，维生素C清除DPPH自由基的IC₅₀为0.046 mg/mL；茶树菇对清除羟基自由基的清除能力最强，其IC₅₀值为1.41 mg/mL，云芝和松茸也有较强清除羟自由基能力，其IC₅₀值分别为1.56、1.57 mg/mL，三者的清除能力均明显优于阳性对照样品，维生素C清除羟自由基的IC₅₀为2.41 mg/mL；灵芝和云芝有较强清除超氧阴离子自由基能力，其IC₅₀值分别为124.48、138.28 mg/mL。

Antioxidant Activity of Water Extraction from Four Rare Edible Mushroom Species

Antioxidant activities in vitro of polysaccharides from Ganoderma lucidum, Coriolus versicolor, Agrocybe cylindracea and Matsutake mushroom, and vitamin C as a positive control. The anti-oxidation of polysaccharides was evaluated by DPPH radical scavenging activities, hydroxyl free radical scavenging activities and superoxide anion scavenging activities. The results showed that four different polysaccharides had certain abilities of antioxidant activities. C. versicolor showed the most potent capacity for scavenging DPPH free radical, IC₅₀ values reaching 1.46 mg/mL, the IC₅₀ values of vitamin C is 0.046 mg/mL; A. cylindracea showed the most potent capacity for scavenging hydroxyl free radical, IC₅₀ values reaching 1.41 mg/mL, the IC₅₀ values of Vitamin C is 2.41 mg/mL; C. versicolor and Matsutake mushroom also had good scavenging activities, IC₅₀ values reaching 1.56 mg/mL, 1.57 mg/mL, respectively. They were better scavenging activities than positive control. G. lucidum and C. versicolor also had good superoxide anion scavenging activities, IC₅₀ values reaching 124.48 mg/mL, 138.28 mg/mL.