| EBNA3C与Gemin3两种蛋白形成复合物及相互作用的结构域 |
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| 引用本文： | 郭毅,唐艳君,赵楠.EBNA3C与Gemin3两种蛋白形成复合物及相互作用的结构域[J].微生物学杂志,2014(4):27-32. |
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| 作者姓名： | 郭毅 唐艳君 赵楠 |
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| 作者单位： | 中国医科大学附属第一医院 妇科，辽宁 沈阳 110001,鞍山市岫岩满族自治县第三人民医院 妇产科，辽宁 鞍山 114300,中国医科大学附属第一医院 妇科，辽宁 沈阳 110001 |
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| 基金项目： | 国家自然科学基金（81171649）；辽宁省自然科学基金（201102267） |
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| 摘 要： | 探讨EB病毒核抗原3C(EBNA3C)与Gemin3的相互结合及其作用区域。用Flag-EBAN3C与GFPGemin3共转染HEK 293细胞,采用免疫共沉淀、谷胱苷肽-S转移酶共沉淀及免疫荧光蛋白共存实验确定EBNA3C与Gemin3两种蛋白在体内、体外相互结合及相互作用的结构域。EBNA3C与Gemin3两种蛋白在体内外相结合,二者通过各自的C端相互结合。EBNA3C与Gemin3两种蛋白形成稳定复合物。
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| 关 键 词： | EBNA3C Gemin3 结构域 |
Compound Formed by Two Proteins EBNA3C & Gemin3 and Their Interaction Structural Domain |
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| GUO Yi,TANG Yan-jun and ZHAO Nan.Compound Formed by Two Proteins EBNA3C & Gemin3 and Their Interaction Structural Domain[J].Journal of Microbiology,2014(4):27-32. |
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| Authors: | GUO Yi TANG Yan-jun and ZHAO Nan |
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| Institution: | Dept. of Gynecol., 1st Affil. Hosp. of China Med. Uni., Shengyang 110001,Dept. of Gyneacol. & Obstetrics, 3rd People''s Hosp. Manzhu Autonom. County of Xiuyan, Anshan City 114300 and Dept. of Gynecol., 1st Affil. Hosp. of China Med. Uni., Shengyang 110001 |
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| Abstract: | Interaction and working domain of nucleo antigen 3C of EB virus (EBNA3C) with Gemin3 was investigated. HEK 293 cells were co-infected with Flag-EBNA3C and GFP-Gemin3, and their structural domain of two proteins EBNA3C and Gemin3 inter-conjunction and interaction in vivo and in vivo was confirmed by co-existence experiment adopting immune co-precipitation, glutathione-S transferase and immunofluorescence protein. EBNA3C and Gemin3 combined with each other in vivo and in vivo through their individual C-terminal. Two kinds of proteins of EBNA3C and Gemin3 formed a stable compound. |
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| Keywords: | EBNA3C Gemin3 structural domain |
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