| 稻曲病菌厚垣孢子壁黑色素提取方法研究 |
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| 引用本文： | 毛莹,任佐华,王娜,白珍安,虞选杰,刘二明.稻曲病菌厚垣孢子壁黑色素提取方法研究[J].微生物学杂志,2011,31(1):6-9. |
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| 作者姓名： | 毛莹 任佐华 王娜 白珍安 虞选杰 刘二明 |
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| 作者单位： | 湖南农业大学生,物安全科学技术学院,湖南,长沙,410128 |
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| 摘 要： | 探究稻曲病菌（Ustiloginoidea virens（Cooke） Takahashi）厚垣孢子壁黑色素的最佳提取方法,采用以HCl为提取剂的酸提法和以NaOH为提取剂的碱提法,对该病菌黑色和黄色2种厚垣孢子壁的黑色素进行提取,用3因素3水平进行正交设计试验,结果表明以NaOH作提取剂为佳,其提取黑色素效果最佳的组合条件为3 mol/L NaOH、2 mol/L HC l、水浴温度80℃、水浴时间120 min。
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| 关 键 词： | 稻曲病菌 厚垣孢子壁 黑色素 提取 正交设计 |
Melanin Extraction from Chlamydospore Wall in Ustiloginoidea virens |
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| MAO Ying,REN Zuo-hu,WANG N,BAI Zhen-an,YU Xuan-jie,LIU Er-ming.Melanin Extraction from Chlamydospore Wall in Ustiloginoidea virens[J].Journal of Microbiology,2011,31(1):6-9. |
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| Authors: | MAO Ying REN Zuo-hu WANG N BAI Zhen-an YU Xuan-jie LIU Er-ming |
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| Institution: | MAO Ying,REN Zuo-hua,WANG Na,BAI Zhen-an,YU Xuan-jie,LIU Er-ming(Coll.of Bio-Safety Sci.& Technol.,Hunan Agric.Uni.,Changsha 410128) |
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| Abstract: | HCl as an extractant for acid extraction and NaOH as an extractant for alkaline extraction were adopted to study the optimum extraction of melanin from chlamydospore wall in Ustilaginoidea virens,melanin extraction of black and yellow chlamydospore wall in U.virens and the experiment was carried out with orthogonal design using 3 factors and 3 levels.The result showed that using NaOH as the extractant was of optimal,and the optimal combination conditions was 3 mol/L NaOH,2 mol/L HCl in 80 ℃ water bath for 120 min. |
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| Keywords: | Ustiloginoidea virens chlamydospore wall melanin extraction orthogonal design |
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