| 一种金针菇核糖体失活蛋白的分离纯化研究 |
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| 引用本文： | 孙宇峰,于德水,奚新伟,沙长青,陈静宇,陈菠,杨忠亮,杨志兴.一种金针菇核糖体失活蛋白的分离纯化研究[J].微生物学杂志,2007,27(4):62-65. |
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| 作者姓名： | 孙宇峰 于德水 奚新伟 沙长青 陈静宇 陈菠 杨忠亮 杨志兴 |
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| 作者单位： | 1. 黑龙江省科学院,应用微生物研究所,黑龙江,哈尔滨,150010
2. 哈尔滨医药集团,生物工程制药厂,黑龙江,哈尔滨,150020 |
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| 基金项目： | 黑龙江省科技攻关重点研究项目 |
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| 摘 要： | 获得一种为研究其他菌类核糖体失活蛋白的对照品,并论述一种金针菇核糖体失活蛋白的分离纯化及其活性的研究结果。实验中采用了DEAE和CM-离子交换纤维素与Bio-Gel 100柱层析方法。从1 000 g新鲜金针菇中得到5.58 mg的核糖体失活蛋白-Velutin,并证明其具有明显的抑制蛋白质的翻译作用,同时简要介绍了它的应用及展望。分离纯化到具有活性的Velutin,分子量为13.8 ku。
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| 关 键 词： | 金针菇 核糖体失活蛋白 家兔网织红细胞裂解系统 抑制蛋白质的翻 |
| 文章编号： | 1005-7021(2007)04-0062-04 |
| 修稿时间： | 2007年1月23日 |
The Study on Isolation and Purifacation of One Ribosome Inactivating Protein from Flammulina velutipes |
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| SUN Yu-feng,YU De-shi,XI Xin-wei,SHA Chang-qing,CHEN Jing-yu,CHEN Bo,YANG Zhong-liang,YANG Zhi-xing.The Study on Isolation and Purifacation of One Ribosome Inactivating Protein from Flammulina velutipes[J].Journal of Microbiology,2007,27(4):62-65. |
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| Authors: | SUN Yu-feng YU De-shi XI Xin-wei SHA Chang-qing CHEN Jing-yu CHEN Bo YANG Zhong-liang YANG Zhi-xing |
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| Abstract: | In order to gain a ribosome inactivating protein(RIP) from Flammulina velutipes as controlling preparetion in reseach of RIP of other fungi,this paper discribed a study on isolation and purification of one RIP from Flammulina velutipes.In the study,DEAE-cellulose,CM-cellulose and Bio-gel 100 column chromatographs was used.5.58 mg RIP(Velutin) was extracted from 1 000 g Flammulina velutipes.Which exhibited evident inhibition to translation of protein in Rabbit reticulocyte lysate.The applications and prospects were also reviewed briefly.An activated RIP-Velutin was isolated and purified with a molecular weight of 13.8 ku. |
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| Keywords: | Flammulina velutipes Velutin Ribosome-Inactivating Proteins Inhibition of protein translation Rabbit reticulocyte lysate |
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