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| 核酸疫苗双表达载体的构建及表达 | |
| 引用本文： | 赵大鹏,吴晓娟,戚凤春,冷梅,张岩,汪春义,张雪梅,陈云波,何伟,任清,付学奇.核酸疫苗双表达载体的构建及表达[J].微生物学杂志,2006,26(5):37-39. |
| 作者姓名： | 赵大鹏 吴晓娟 戚凤春 冷梅 张岩 汪春义 张雪梅 陈云波 何伟 任清 付学奇 |
| 作者单位： | 1. 长春生物制品研究所,吉林,长春,130062 2. 长春工业大学,生物工程学院,吉林,长春,130012 3. 吉林大学,生命科学学院,吉林,长春,130021 |
| 摘 要： | 将微小病毒内部核糖体进入位点(IRES)基因克隆到质粒pVAXI载体多克隆位点,构建出核酸疫苗双表达载体pVI。将绿色荧光蛋白(EGFP)基因和新霉素磷酸转移酶(neor)基因作为报告基因,连接到pVI载体IRES基因的前后两处多克隆位点,构建出表达载体pEIN。通过脂质体介导的方法将该载体转染COS-7细胞,筛选到同时表达绿色荧光蛋白和新霉素磷酸转移酶的表达株,表明成功地构建了核酸疫苗双表达载体,为构建多价核酸疫苗及带有分子佐剂的核酸疫苗打下了基础。 |
| 关 键 词： | IRES EGFP neor 转染 |
| 文章编号： | 1005-7021(2006)05-0037-03 |
| 修稿时间： | 2006年7月20日 |
Construction and Expression of Co-Expression Vector for DNA Vaccine |
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| ZHAO Da-peng,WU Xiao-juan,QI Feng-chun,LENG Mei,ZHANG Yan,WANG Chun-yi,ZHANG Xue-mei,CHEN Yun-bo,HE Wei,REN Qing,FU Xue-qi.Construction and Expression of Co-Expression Vector for DNA Vaccine[J].Journal of Microbiology,2006,26(5):37-39. | |
| Authors: | ZHAO Da-peng WU Xiao-juan QI Feng-chun LENG Mei ZHANG Yan WANG Chun-yi ZHANG Xue-mei CHEN Yun-bo HE Wei REN Qing FU Xue-qi |
| Abstract: | Internal ribosome entry site(IRES) gene was cloned into plasmid pVAXI to construct a eukaryotic co-expression plasmid pVI for DNA vaccine.Then enhanced green fluorescent protein(EGFP) gene and neomycin phosphotransferase(neo~r) gene fragment were ligated with pVI as reporter genes into its two multiple cloned sites by IRES respectively to construct co-expression plasmid pEIN containing two expression units of EGFP and neor.Recombinant vector was transfected into COS7 cell by means of lipid mixtures.The cell expressing the two proteins was obtained by screening.The successful construction of the eukaryotic co-expression vector became the foundation for multivalency DNA vaccine and DNA vaccine containing molecular adjuvant. |
| Keywords: | IRES EGFP neo~r transfection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |