| L-蛋氨酸酰化酶基因工程菌培养条件的研究 |
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| 引用本文： | 李环,柏中中,何学军,韦萍.L-蛋氨酸酰化酶基因工程菌培养条件的研究[J].微生物学杂志,2003,23(5):25-27,30. |
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| 作者姓名： | 李环 柏中中 何学军 韦萍 |
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| 作者单位： | 南京工业大学,制药与生命科学学院,江苏,南京,210009 |
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| 摘 要： | 为提高L-氨基酰化酶基因工程菌1016的生物量及酶产量,优化了发酵工艺,确定了较佳的培养基成分,并初步考察了该酶的底物特异性。优化后工程菌摇瓶产酶稳定在980U/mL发酵液,发酵周期17 h,生物量A_(420)穗定在0.7。产酶量为原来的2倍以上。乙酰化的脂肪族氨基酸Met是该酶的最适底物。
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| 关 键 词： | 氨基酰化酶 基因工程菌 蛋氨酸 发酵 |
| 文章编号： | 1005-7021(2003)05-0025-03 |
| 修稿时间： | 2002年10月28 |
Study on The Culture of Engineering Bacteria 1016 Producing L-aminoacylase |
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| LI Huan,BAI Zhongzhong.HE Xuejun.WEI Ping.Study on The Culture of Engineering Bacteria 1016 Producing L-aminoacylase[J].Journal of Microbiology,2003,23(5):25-27,30. |
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| Authors: | LI Huan BAI ZhongzhongHE XuejunWEI Ping |
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| Abstract: | In order to improve the biomass and enzyme production of engineering bacteria 1016 which produce L-aminoacy-lase, a suitable media has been found and some fermentation conditions were optimized. Enzyme production of engineering bacteria 1016 maintained 980U/mL in flask. The biomass A420 kept 0.7.The fermentation periods was 17hours. The optical substrate for aminoacylase were N-AC-D,L-Met. |
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| Keywords: | Aminoacylase Engineering bacteria L-methionine Fermentation |
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