| 优化的细菌鞭毛染色技术 |
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| 引用本文： | 王桂萍,朱陶,王宜磊,张子宇,李财新,杨宏宇.优化的细菌鞭毛染色技术[J].微生物学杂志,2007,27(4):94-96. |
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| 作者姓名： | 王桂萍 朱陶 王宜磊 张子宇 李财新 杨宏宇 |
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| 作者单位： | 菏泽学院,生命科学系,山东,菏泽,274015 |
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| 摘 要： | 优化了实验教材上传统的银染液鞭毛染色方法,用单宁酸和FeCl3做媒染剂,增大单宁酸和FeCl3的质量浓度(并将其配制的溶液分别保存),然后用碱性染料沙黄水溶液1]、齐氏石炭酸碱性复红染液1]和稀释10倍吕氏碱性美蓝染液1],分别对培养好的枯草芽胞杆菌进行染色,得到较粗、清晰的染色结果。
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| 关 键 词： | 细菌鞭毛 染色 媒染剂 碱性染料 |
| 文章编号： | 1005-7021(2007)04-0094-03 |
| 修稿时间： | 2007年1月15日 |
An improved staining method for bacterial flagellum |
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| WANG Gui-ping,ZHU Tao,WANG Yi-lei,ZHANG Zi-yu,LI Cai-xin,YANG Hong-yu.An improved staining method for bacterial flagellum[J].Journal of Microbiology,2007,27(4):94-96. |
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| Authors: | WANG Gui-ping ZHU Tao WANG Yi-lei ZHANG Zi-yu LI Cai-xin YANG Hong-yu |
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| Abstract: | This study is to optimze the traditional SilverQuest staining method.By using tannin and FeCl3 with higher mass concentrations as mordant(these solutions were separately stored),a stable and clear staining result of Bacillus subtitles was obtained which respectively stained by safranin aqueous solution,the Qi's carbolic acid alkalescence fuchsin and decuple diluted lv's alkalescence methylene blue. |
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| Keywords: | Bacterial flagellum Staining Mordant Basic dye |
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