儿童口腔内变形链球菌、乳酸杆菌的检出情况分析

调查评估年龄<12岁儿童口腔内变形链球菌及乳酸杆菌的检出情况及其与龋病的相关性。100例纳入实验的受试者根据不同龋敏感程度分为无龋组(DMFS=0)50人,高龋组(DMFS≥6)50人,按照不同龋敏感度、不同部位采集样本,用乳酸杆菌选择性培养基Rogosa和变形链球菌选择性培养基MSB,将乳酸杆菌和变形链球菌分离,经形态学及生化鉴定。统计2种细菌在不同分组中的检出率及不同致龋菌病例数,研究二者的致龋相关性。乳酸杆菌在100例研究对象中乳酸杆菌在龋坏深层检出率最高76%与光滑面(40%)、窝沟(48%)及唾液(40%)检出率比较有统计学意义(P<0.05),而龋坏深层变形链球菌检出率46%与光滑面(84%)、窝沟(68%)及唾液(78%)检出率比较最低(P<0.05);高龋人群变形链球菌检出率88%,乳酸杆菌检出率为76%均较无龋组相应细菌检出率26%、28%差异有统计学意义(P<0.05)。变形链球菌和乳酸杆菌随着龋敏感度的增加,乳酸杆菌和变形链球菌检出率增高;在龋坏深层更适于乳酸杆菌生长,且其对变形链球菌有一定的抑制作用。

Analysis of Tested Streptococcus mutans and Lactobacillus Inside the Children's Mouth

The reciprocity between tested Streptococcus mutans as well as Lactobacillus from children＇s mouth under twelve and carious disease was investigated and valued.100 cases of testees were divided into carious-active group（DMFS≥6）,carious-free group（DMFS=0） according to DMFS（surface of decayed missing filled）,and collected samples from different parts.And morpholigically and biochemically identified Lactobacillus adopting selective Rogosa medium and S.mutans adopting selective MSB medium to isolate Lactobacillus and S.mutans.Then counted up the tested rates of both bacteria in different groups and the case numbers of carious disease to study the reciprocity of cario-genesis of both.The results showed that the tested rate of Lactobacillus in 100 cases of studying targets was 76% from caries lesion,40% from smooth surface,48% from pits and fissures,and 40% from saliva,and the tested rates were statistically significant（P〈0.05）.While the tested rate of S.mutans was 46% from caries lesion,it was the lowest（P〈0.05） as compared with those from smooth surface（84%）,from pits and fissures（68%）,and from saliva（78%）.The tested rate of S.mutans in highly carious press of people was 88%,Lactobacillus was 76%,both tested rate of homologized bacteria were 26% and 28%,the differences were statistically significant（P〈0.05） as compared with carous-free group.Therefore,the tested rate of S.mutans and Lactobacillus increased as the increment of carious sensitivity.The caries lesion was more suitable for the Lactobacillus to grow,and they had inhibition to some extent against S.mutans.