| 利用气相色谱法同时测定土壤中反硝化作用和固氮作用的强度 |
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| 引用本文： | 胡连生,商曙辉,王淑芳,陈冠雄,吴劼.利用气相色谱法同时测定土壤中反硝化作用和固氮作用的强度[J].微生物学杂志,1992(2). |
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| 作者姓名： | 胡连生 商曙辉 王淑芳 陈冠雄 吴劼 |
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| 作者单位： | 中国科学院沈阳应用生态研究所 110015 |
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| 摘 要： | 土壤中反硝化菌和固氮菌的作用强度与其产生的N_2O及C_2H_2还原成C_2H_4的量有一定关系。本文报导了为测定土壤中微量的N_2O、C_2H_2和C_2H_2数量,所建立的双柱双检测器一次进样同时测定的二维气相色谱法。此法能简单、快速、准确地反映出土壤中反硝化菌和固氮菌的作用强度。文中对仪器装置、色谱条件、样品处理、干扰物的消除、最小检测浓度、重复性、方法应用等进行了报导。
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| 关 键 词： | 气相色谱法 反硝化菌 固氮菌 作用强度 二维气相色谱法 |
SIMULTANEOUS DETERMINATION OF INTENSITY OF THE DENITRIFICATION AND NITROGEN FIXATION IN SOIL BY GAS CHROMATOGRAPHY |
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| Hu Liansheng Shang shuhui Wang Shufang Chen Guanxiong Wu Jie.SIMULTANEOUS DETERMINATION OF INTENSITY OF THE DENITRIFICATION AND NITROGEN FIXATION IN SOIL BY GAS CHROMATOGRAPHY[J].Journal of Microbiology,1992(2). |
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| Authors: | Hu Liansheng Shang shuhui Wang Shufang Chen Guanxiong Wu Jie |
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| Abstract: | In this paper,a simultaneous determination of trace amount of N_2O and C_H_4/C_2H_2 produced by the denitrification and nitrogen fixation in soil by two dimensional gas chromatography of dual column and dual detector was presented. A simple, fast and accurate analytical method was developed. Instrumental installation, column, conditions, sample disposal, interfering component elimination, minimum detectable concentration, repeatability and applications cf the method were reported. |
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| Keywords: | Gas Chromatography Denitrobacteria Azotobacteria Action Intensity Two Dimensional Gas chromatography |
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