| 化学诱变法选育D-核糖高产菌株工艺研究 |
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| 引用本文： | 陈新征,吴兆亮,赵静波,胡滨.化学诱变法选育D-核糖高产菌株工艺研究[J].微生物学杂志,2005,25(2):107-109. |
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| 作者姓名： | 陈新征 吴兆亮 赵静波 胡滨 |
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| 作者单位： | 河北工业大学,生物工程系,天津,300130 |
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| 基金项目： | 河北省自然基金(201010)，河北工业大学“211工程”资助项目 |
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| 摘 要： | 研究了以硫酸二乙酯(DES)为诱变剂,诱变生产D-核糖的转酮醇酶缺陷型枯草芽孢杆菌HG02,考察了不同诱变剂用量对菌体致死率及其生产能力的影响。得出了以DES诱变该菌株的致死率曲线及最佳的诱变条件:诱变剂用量0.8%,诱变时间15min。该诱变条件下对大量的突变株进行筛选,得到D-核糖高产菌HG03,其D核糖产量较出发菌株提高81.69%。达到5.1g/100mL。
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| 关 键 词： | 硫酸二乙酯 枯草芽孢杆菌 诱变 致死率 |
| 文章编号： | 1005-7021(2005)02-0107-03 |
| 修稿时间： | 2003年11月28 |
Breeding Technology of D-Ribose High-Producing Bacillus Strain with Chemical Mutation |
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| CHEN Xin-zheng,WU Zhao-liang,ZHAO Jing-bo,HU Bin.Breeding Technology of D-Ribose High-Producing Bacillus Strain with Chemical Mutation[J].Journal of Microbiology,2005,25(2):107-109. |
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| Authors: | CHEN Xin-zheng WU Zhao-liang ZHAO Jing-bo HU Bin |
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| Institution: | Dept. of Biotech. Hebei Univ. of Technol. Tianjin 300130 |
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| Abstract: | A transketolase-defective Bacillus subtilis strains HG02 was treated with diethylsulfate (DES) to obtain D-ribose producing strain. Lethal rate of different DES dosages and the effects on production capacity were observed and obtained a lethal curve of the strain and optimum conditions of the mutation, they are mutation dosage at 0.8% and mutation time for 15 minutes. Under such mutation conditions a large number of mutants was screened and finally obtained D-ribose high-producing strain HG03. The output of D-ribose increased by 81.69% as compared with the starting strain and hit up to 5.1 g/100mL. |
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| Keywords: | diethylsulfate Bacillus subtilis mutagenesis lethal rate |
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