乳酸杆菌基因表达系统的研究进展

乳酸杆菌是人及动物肠道中重要的益生菌,被公认为安全级(generally recognized as safe,GRAS)微生物。乳酸杆菌表达系统是近几年发展起来的一种表达系统,随着乳酸杆菌分子生物学的发展、各类表达调控元件的分离,相继发展了乳酸杆菌的克隆载体、表达载体和整合载体。乳酸杆菌具有免疫佐剂、吸附黏膜、抗胆汁酸能力,以乳酸杆菌为载体,作为外源基因的传递和表达系统,研制口服疫苗,刺激黏膜免疫系统产生有效的免疫应答,具有重要开发前景。

Progress on Gene Expression System of Lactobacillus

Lactobacillus is a kind of important probiotic in gastro-intestinal tracts of human and most animals,and they are considered to be safe bacteria with a GRAS(generally regarded as safe) status.The expression systems of Lactobacillus is a kind of expression system that have been developed in recent years,and as the development of molecular biology of Lactobacillus,and the separation of various expression regulatory elements,the cloning vector,the expression vector,and the integration vector have been developed one after another.Lactobacillus possesses many properties such as immune adjuvant,adsorption mucosa,anti-bile acid capability;take Lactobacillus as a vector transferor and expression systems of exogenous gene to study oral vaccine,stimulate mucosa immune system to produce effective immune responses,all of these possess important development prospect.