| 土壤放线菌FX05发酵培养基及发酵条件的优化 |
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| 引用本文： | 夏觅真,蒋颂,罗薇薇,尹帅,曹伟,陈首慧,薛绍礼,王怡.土壤放线菌FX05发酵培养基及发酵条件的优化[J].微生物学杂志,2009,29(4):49-52. |
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| 作者姓名： | 夏觅真 蒋颂 罗薇薇 尹帅 曹伟 陈首慧 薛绍礼 王怡 |
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| 作者单位： | 安徽医科大学,医学生物工程教研室,安徽,合肥,230032 |
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| 摘 要： | 以高氏1号培养基为基础，通过单因素分析和正交试验，对土壤放线菌FX05最佳培养基扣最优培养条件进行了研究。结果表明：适宜FX05产抑绿脓杆菌活性物质的发酵培养基配方为玉米粉1％、NaNO30．3％、K2HPO40．075％、MgS040．075％，发酵条件为初始pH为7．0，培养温度为28℃，培养96h产抑菌物质迭最大值。
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| 关 键 词： | 土壤放线菌 活性物质 培养条件优化 |
Optimization of Medium and Fermentation Conditions for Soil Actinomyces Strain FX05 |
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| XIA Mi-zhen,JIANG Song,LUO Wei-wei,YIN Shuai,CAO Wei,CHEN Shou-hui,XUE Shao-li,WANG YI.Optimization of Medium and Fermentation Conditions for Soil Actinomyces Strain FX05[J].Journal of Microbiology,2009,29(4):49-52. |
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| Authors: | XIA Mi-zhen JIANG Song LUO Wei-wei YIN Shuai CAO Wei CHEN Shou-hui XUE Shao-li WANG YI |
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| Institution: | (Dept. of Med. Biotechnol. , Anhui Med. Univ. , Hefei 230032) |
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| Abstract: | Based on Gause＇ s No 1 synthetic medium, optimization of medium and fermentation conditions for soil Actinomyces strain FX05 was studied by single factor analysis and orthogonal experiment. The results showed that medium with 1% of corn meal, 0.3% of NaNO3 , 0. 075 % of MgSO4 , starting pH at 7.0, culture temperature at 28 ℃ for 96 hours, the production of inhibition substance reached to the maximum |
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| Keywords: | soil Actinomyces active substance optimization of culture conditions |
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