| 黑暗链霉菌中tbmA基因的功能研究 |
| |
| 引用本文： | 杨钧,夏焕章,余永红,刘晓辉.黑暗链霉菌中tbmA基因的功能研究[J].微生物学杂志,2007,27(4):31-34. |
| |
| 作者姓名： | 杨钧 夏焕章 余永红 刘晓辉 |
| |
| 作者单位： | 沈阳药科大学,生物制药系,辽宁,沈阳,110016 |
| |
| 摘 要： | PCR获得tbmA基因内部863 bp片段,构建基因阻断穿梭载体pSPU112-1,经接合转移导入Strepto-myces tenebrariusH6,筛选单交换阻断变株,并用Southern blot验证阻断变株的tbmA已经被破坏。经发酵产物分析,阻断变株不再合成氨甲酰妥布霉素,只合成安普霉素。首次从分子水平证明了tbmA只参与氨甲酰妥布霉素生物合成,而不参与安普霉素的生物合成。
|
| 关 键 词： | 黑暗链霉菌 氨甲酰妥布霉素 生物合成基因 基因阻断 |
| 文章编号： | 1005-7021(2007)04-0031-04 |
| 修稿时间： | 2007年3月26日 |
Functional analysis of tbmA gene of Streptomyces tenebrarius |
| |
| YANG Jun,XIA Huan-zhang,YU Yong-hong,LIU Xiao-hui.Functional analysis of tbmA gene of Streptomyces tenebrarius[J].Journal of Microbiology,2007,27(4):31-34. |
| |
| Authors: | YANG Jun XIA Huan-zhang YU Yong-hong LIU Xiao-hui |
| |
| Abstract: | A 863 bp amplification fragment of tbmA gene of Streptomyces tenebrarius was obtained by PCR.A recombinant plasmid pSPU112-1 containing the 863 bp fragment for gene blocking was constructed and then the plasmid was transfered into S.tenebrarius H6 by way of conjugation.The result of southern blot assay indicated that the tbmA gene of the blocked mutant was exactly disrupted.The mutant could synthesize apramycin nut no longer produced carbamoyltobramycin,which is firstly confirmed that tbmA gene is closely related to carbamoyltobramycin biosynthesis but not involved in apramycin biosynthesis. |
| |
| Keywords: | S tenebrarius Carbamoyltobsamycin Biosynthesis genes Gene disruption |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
