| 1株聚乳酸降解细菌的筛选、鉴定及产酶研究 |
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| 引用本文： | 刘玲绯,李凡,林秀梅,刘东波,夏红梅,陈珊.1株聚乳酸降解细菌的筛选、鉴定及产酶研究[J].微生物学杂志,2011,31(5):7-13. |
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| 作者姓名： | 刘玲绯 李凡 林秀梅 刘东波 夏红梅 陈珊 |
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| 作者单位： | 东北师范大学生命科学学院,吉林长春,130024 |
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| 基金项目： | 国家自然科学基金(31100046):吉林省科技发展计划项目，中央高校基本科研业务费专项资金资助项目 |
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| 摘 要： | 从污泥中筛选出1株对聚乳酸（poly—L—lacticacid，PLA）具有降解活力的细菌DSL09，该菌株对PLA的乳化液、粉末及薄膜都具有降解作用。通过形态学、16SrDNA比对及生理生化特性的分析，鉴定该菌株属于芽胞杆菌属（Bacillus sp．）。为提高该菌株对PLA的降解活力，对其进行了紫外诱变，获得了稳定遗传的突变株DSL09-60b，该突变株的PLA降解活性提高至原始菌株的1．5倍。对该突变株产PLA降解酶的发酵条件进行了优化，经测定DSL09-60b在初始培养基pH为8．0、0．5％酪蛋白为诱导物、接种量6％（体积比）的条件下37℃培养54h时发酵液酶活性最高。
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| 关 键 词： | 聚乳酸 芽胞杆菌 紫外诱变 PLA降解酶 |
Isolation, Identification and Enzyme Producing Conditions of Poly-L-lactic Acid (PLA)-degrading Bacillus sp.Strain DSL09 |
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| LIU Ling-fei,LI Fan,LIN Xiu-mei,LIU Dong-bo,XIA Hong-mei,CHEN Shan.Isolation, Identification and Enzyme Producing Conditions of Poly-L-lactic Acid (PLA)-degrading Bacillus sp.Strain DSL09[J].Journal of Microbiology,2011,31(5):7-13. |
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| Authors: | LIU Ling-fei LI Fan LIN Xiu-mei LIU Dong-bo XIA Hong-mei CHEN Shan |
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| Institution: | LIU Ling-fei,LI Fan,LIN Xiu-mei,LIU Dong-bo,XIA Hong-mei,CHEN Shan(Life Sci.,NE Normal Uni.,Changchun,Jilin 130024) |
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| Abstract: | A strain possessing activity to degrade poly-L-lactic acid(PLA) DSL09 was screened from sludge.The strain had the effect to degrade PLA in emulsion,powder or film.It was identified as Bacillus sp.by the analyses of morphology,16S rDNA comparison,physiological and biochemical characteristics.In order to enhance the degradation activity it was mutated with UV and obtained a genetically stable mutant strain DSL09-60b which increased PLA-degrading activity by 1.5 times of the original strain.The fermentation co... |
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| Keywords: | poly-L-lactic acid(PLA) Bacillus sp ultraviolet mutation PLA degradase |
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