肠道菌群对蛋氨酸脑啡肽治疗肿瘤疗效的影响

蛋氨酸脑啡肽(Methionine enkephalin, MENK)是一种内源性阿片肽,有良好的抗肿瘤作用,而肠道菌群对MENK抗肿瘤作用的影响尚不清楚。建立小鼠肠道菌群紊乱模型及荷瘤小鼠模型,以16S rRNA测序法检测肠道菌群丰度、监测小鼠体重、绘制肿瘤生长曲线,以流式细胞术检测小鼠肿瘤浸润CD4~+T细胞、CD8~+T细胞、Treg细胞百分比。结果表明,小鼠肠道菌群紊乱模型建立成功;肠道菌群正常与紊乱荷瘤小鼠同时接受MENK治疗,后者肿瘤体积更大,菌群正常荷瘤小鼠肿瘤浸润的CD4~+T细胞、CD8~+T细胞百分比上调,Treg细胞百分比下调,而菌群紊乱荷瘤小鼠与菌群正常荷瘤小鼠相比,肿瘤浸润CD4~+T细胞、CD8~+T细胞百分比下调,Treg细胞百分比上调。该研究表明肠道菌群紊乱可以降低MENK治疗肿瘤的疗效。

Influence of Intestinal Microbial Population on Therapeutic Effect of Methionine Enkephalin on Tumor

Methionine enkephalin (MENK) is an endogenous opioid peptide that has good antitumor effects, while the influence of intestinal microbial population (IMP) on the anti-tumor effect of MENK is still unclear. In this study, a mouse IMP disorder model and a tumour-bearing mouse model were established to detect the IMP abundance by 16S rRNA sequencing method, to monitor the weight of mice, and draw tumor growth curve, and detect the percentage of CD4⁺T cells, CD8⁺T cells and Treg cells infiltrated by mice tumor by flow cytometry. The results showed that the model of IMP disturbance in mice was established successfully. Both tumor-bearing mice with normal IMP and tumor-bearing mice with disturbed IMP were treated with MENK, the latter had a larger tumor volume, the percentage of CD4⁺T cells and CD8⁺T cells infiltrated by tumor in tumor-bearing mice with normal IMP was up-regulated, Treg cells percentage was down-regulated, when tumor-bearing mice with disordered IMP were compared with tumor-bearing mice with normal IMP, the percentage of CD4⁺T cells and CD8⁺T cells infiltrated by tumor in tumor bearing mice was down-regulated, Treg cells percentage was up-regulated. This study showed that IMP disturbance could reduce the efficacy of MENK in treatment of tumors.