应用分子生物学方法检测宫颈液基细胞残液中HPV16／18感染的对比研究

对比免疫蛋白印迹及斑点杂交检测人乳头瘤病毒HPV16/18的检出率,以期找到子宫颈癌早期筛查的最佳方法。采用免疫蛋白印迹与斑点杂交分别对173份宫颈液基细胞进行HPV16/18检测。150例经宫颈液基细胞学镜下筛查为宫颈上皮内病变及宫颈癌的患者,其中免疫蛋白印迹对低度病变检出率为47.6%(30/63),对高度病变检出率为57.9%(33/57),对浸润癌的检出率为86.7%(26/30),其中鳞癌为85%(17/20),腺癌为90%(9/10),采用斑点杂交对低度病变检出率为28.6%(18/63),对高度病变检出率为42.1%(24/57),浸润癌的检出率为80%(24/30),其中鳞癌为75%(15/20),腺癌为90%(9/10)。采用免疫蛋白印迹对23例正常对照的细胞HPV检出率为13.1%(3/23),斑点杂交的检出率为8.7%(2/23)。各级宫颈病变中HPV16/18的检出率均较对照组差异有统计学意义(P<0.05)。免疫蛋白印迹检测HPV16/18比斑点杂交的检出率要高,为发现HPV一过性携带者,达到早期诊断的目的。

Comparison of HPV16/18 Infection in Residual Fluid of Cervical Liquid-based Cells Testing Applying Molecular Biological Method

The detection rate of the infection by HPV16/18 with western-blotting and dot-blotting hybridization of immunoprotein was compared in order to discover the best method to screen the early carcinoma of cervix uterus,173 residual fluid of cervical liquid-based cells samples were respectively tested adopting western-blotting and dot-blotting hybridization of immunoprotein,150 cases were diagnosed as LSIL,HSIL,and CC by microscopy,and western-blotting detection of HPV16/18 in LSIL 47.6%(30/63),in HSIL 57.9%(33/57),and in CC86.7%(26/30),including SC 85%(17/20) and AC90%(9/10),dot-blotting detection of HPV16/18 in LSIL 28.6%(2/23),in HSIL 42.1%(24/57),and in CC80%(24/30),including SC 75%(15/20) and AC 90%(9/10),HPV16/18 test with western-blotting in 23 cases of normal cells 13.1%(3/23),while with dot-blotting 8.7%(2/23).And HPV16/18 infection was assessed at all stages of cervical neoplasia samples with statistic significance as compared with the control(P<0.05).Therefore,the detection rate of HPV16/18 with western-blotting hybridization of immunoprotein was higher than that of dot blotting and is good for finding HPV transient carrier to diagnose earlier.