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食源性致病菌多重分子生物学检测技术研究进展
引用本文:索标,汪月霞,艾志录.食源性致病菌多重分子生物学检测技术研究进展[J].微生物学杂志,2010,30(6):71-75.
作者姓名:索标  汪月霞  艾志录
作者单位:[1]河南农业大学食品科学技术学院,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002
基金项目:河南省重点科技攻关计划项目
摘    要:快速、可靠的食源性致病菌高通量检测方法对于确保食品安全具有重要意义,近年基于DNA水平的多重分子生物学检测技术迅速发展,针对各种不同的食源性致病菌建立了多种多重分子检测技术,包括多重PCR、多重实时荧光PCR以及基因芯片等。对这些多重分子检测技术的最新研究进展作一综述,并且建议在今后该技术的研究中,仍需要在食品中多种致病菌同时选择性增菌培养、亚致死损伤修复以及检测内标的构建等方面取得突破,从而能够更好地实现食源性致病菌的高通量检测。

关 键 词:多重分子方法  食源性致病菌  多重PCR  基因芯片  检测

Research Development on Multiplex Molecular Detection Methods of Foodborne Pathogens
SUO Biao,WANG Yue-xi,AI Zhi-lu.Research Development on Multiplex Molecular Detection Methods of Foodborne Pathogens[J].Journal of Microbiology,2010,30(6):71-75.
Authors:SUO Biao  WANG Yue-xi  AI Zhi-lu
Institution:1.Coll.of Food Sci.& Technol.,2.Coll.of Life Sci.,Henan Agric.Uni.,Zhengzhou,450002)
Abstract:Rapid,dependable and high throughput methods to detect and characterize foodborne pathogens is of great significance for food safety.Recently multiplex molecular methods based on molecular biology at DNA level have been developed rapidly,and established various multiplex molecular testing techniques including multiplex PCR,multiplex real-time fluorescence PCR and gene chip etc.The newest research progress was reviewed in this article,suggested that a breakthrough on the aspects of simultaneous selective enrichment,recovery of sublethal injured cells and construction of internal control in foods are still needed accordingly to serve for a better realization of high throughput detection of foodborne pathogens.
Keywords:multiplex molecular methods  foodborne pathogens  multiplex PCR  gene chip  detection
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