产脲酶芽胞杆菌的微波诱变育种

巴氏芽胞杆菌是目前微生物诱导碳酸钙沉淀(MICP)方法中应用最为热门的一种细菌。为提高巴氏芽胞杆菌尿素分解以及矿化能力,以巴氏芽胞杆菌YB-B为出发菌株,采用微波诱变育种技术,通过诱变菌株特性筛选及其遗传稳定性检测,成功选育出2株突变菌株YB-3和YB-4。与出发菌株相比,诱变菌株尿素分解能力较原菌株提高1.5倍左右,矿化能力提高114%。诱变菌株具有生长速度快,环境适应性强,矿化能力高等优点,这为MICP更广层次的应用奠定了坚实的基础。

Microwave Mutation Breeding for Urease Producing Sporoscarcina pasteurii

Sporoscarcina pasteurii is known as the most popular bacterium applied to microbial induced calcium carbonate precipitation (MICP) method in the present researches. In order to improve its ability of urea decomposition and biomineralization, two mutant strains YB-3 and YB-4 has been successfully bred based on the S. pasteurii strain YB-B adopting microwave breeding technique, and determined and tested their genetic stability through special screening of the mutant strains. The capacity of urea decomposition had been increased 1.5 times or so and biomineralization by 114% respectively as compared with the starting strain YB-B. Moreover, the mutant strains have been proved possess significant advantages such as fast speed of growth, good environmental adaptability and high mineralization, these had laid a solid foundation for the wider aspect of application of the MICP.