| 中华婪步甲共生放线菌的抗菌活性筛选及菌株BJ37的初步鉴定 |
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| 引用本文： | 孙飞飞,徐晓,陈璐佳,董心怡,王雅娟,向海珍,张桂山,张应烙.中华婪步甲共生放线菌的抗菌活性筛选及菌株BJ37的初步鉴定[J].微生物学杂志,2018,38(3):71-74. |
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| 作者姓名： | 孙飞飞 徐晓 陈璐佳 董心怡 王雅娟 向海珍 张桂山 张应烙 |
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| 作者单位： | 浙江师范大学化学与生命科学学院;中国农业科学院农业资源与农业区划研究所农业部农业微生物资源收集与保藏重点实验室 |
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| 基金项目： | 浙江省自然科学基金项目（LY17C010002）；中央级公益性科研院所基本科研业务费专项(1610132016049)；国家微生物资源平台项目（NIMR） |
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| 摘 要： | 利用平板分离法从中华婪步甲(Harpalus sinicus)中分离得到56株共生放线菌。其中菌株BJ37具有较好的抗菌活性,其发酵液对枯草芽胞杆菌和白色念珠菌的抑菌圈直径均大于30.0 mm。进一步研究BJ37发酵液不同极性溶剂萃取物的抗菌活性,结果表明在中等极性部位存在活性物质。通过形态学特征和分子生物学分析确定该菌株相似菌株为委内瑞拉链霉菌(Streptomyces venezuelae)。菌株BJ37作为微生物杀菌剂潜在产生菌值得进一步研究。
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| 关 键 词： | 中华婪步甲 共生放线菌 分类鉴定 抗菌活性 |
Screening of Antimicrobial Symbiotic Actinomycetes from Harpalus sinicus and Initial Identification of Strain BJ37 |
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| SUN Fei-fei,XU Xiao,CHEN Lu-ji,DONG Xin-yi,WANG Ya-juan,XIANG Hai-zhen,ZHANG Gui-shan and ZHANG Ying-lao.Screening of Antimicrobial Symbiotic Actinomycetes from Harpalus sinicus and Initial Identification of Strain BJ37[J].Journal of Microbiology,2018,38(3):71-74. |
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| Authors: | SUN Fei-fei XU Xiao CHEN Lu-ji DONG Xin-yi WANG Ya-juan XIANG Hai-zhen ZHANG Gui-shan and ZHANG Ying-lao |
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| Abstract: | Fifty six symbiotic actinomycetes strains were isolated from Harpalus sinicus by plate separation method. Among them strain BJ37 showed the fine antimicrobial activity against Bacillus subtilis and Candida albicans with the inhibition zone diameter of more than 30.0 mm. The antimicrobial activity of BJ37 fermented broth extracted with different polar solvent was further studied, and the results showed that the bioactive substances were focused on the parts of medium polarity. BJ37 was confirmed to be similar to Streptomyces venezuelae identified by the morphological features and molecular biology analyses. As a potential microbial microbicide, it is worthy of further study. |
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| Keywords: | Harpalus sinicus symbiotic actinomycetes classification identification antibacterial activity |
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