| RT-PCR一步法检测甲型肝炎活病毒方法的建立 |
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| 引用本文： | 李淑焱,刘大维,田旺,刘令九,薛勇,岳丹,顾丹阳,王信鹏,刘景晔.RT-PCR一步法检测甲型肝炎活病毒方法的建立[J].微生物学杂志,2005,25(4):28-31. |
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| 作者姓名： | 李淑焱 刘大维 田旺 刘令九 薛勇 岳丹 顾丹阳 王信鹏 刘景晔 |
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| 作者单位： | 长春生物制品研究所,吉林,长春,130062 |
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| 摘 要： | 建立灵敏、特异、快速检测甲型肝炎活病毒(HAV)的方法。提取HAV总RNA,选用HAV高保守区为目标区,设计合成一对引物,通过RT-PCR反应扩增其核酸,用琼脂糖凝胶电泳法检测其扩增产物,紫外灯下观察,约200bp处为目标片段,根据观察结果,计算HAV滴度。RT-PCR一步法快速、灵敏、重复性好、特异性强,可用于甲型肝炎活病毒检测。
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| 关 键 词： | RT-PCR 引物 高保守区 甲型肝炎病毒 |
| 文章编号： | 1005-7021(2005)04-0028-04 |
| 修稿时间： | 2005年1月2日 |
Establishment of One-step RT-PCR Detection Method of Live Hepatitis A Virus |
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| LI Shu-yan,LIU Da-wei,TIAN Wang,LIU Ling-jiu,XUE Yong,YUE Dan,GU Dan-yang,WANG Xin-peng,LIU Jing-ye.Establishment of One-step RT-PCR Detection Method of Live Hepatitis A Virus[J].Journal of Microbiology,2005,25(4):28-31. |
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| Authors: | LI Shu-yan LIU Da-wei TIAN Wang LIU Ling-jiu XUE Yong YUE Dan GU Dan-yang WANG Xin-peng LIU Jing-ye |
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| Abstract: | A detection method with sensitive, specific, and rapid for live hepatitis A virus (HAV) was established. Total RNA was extracted from HAV, and select its high conservative region as goal region, and design a double primers to amplify its nucleotide sequences by RT-PCR, the amplified product was detected with agarose gel electrophoresis, and the goal fragment was observed at 200 bp under UV lamp, then titer of HAV was counted. The results showed that this method has high specificity as well rapidness, sensitivity and good repetition. Therefore, RT-PCR method may be used to detect live HAV. |
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| Keywords: | RT-PCR primer high conservative region HAV |
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