汉坦病毒核酸疫苗在小鼠体内表达分布的初步研究

运用生物荧光技术,肌肉免疫接种小鼠含汉坦病毒S抗原基因片段的核酸疫苗,观察重组核酸疫苗在其体内的表达分布。进一步探讨汉坦病毒核酸疫苗的应用前景。扩增纯化已构建好的含有汉坦病毒S抗原基因片段和绿色荧光蛋白(GFP)基因的重组质粒pEGFP/S;免疫接种小鼠胫前肌,观察pEGFP/S在小鼠体内的表达分布。免疫接种3 d后在实验组小鼠肝、肾、脾、肌肉各组织均检测到较强的绿色荧光。重组质粒pEGFP/S能在小鼠的多个组织器官表达,为深入研究汉坦病毒核蛋白和有效的核酸疫苗奠定了基础。

Preliminary Study on Expression Distribution in vivo of Hantaan Viral Nucleic Acid Vaccine in Mice

The expression distribution in vivo of recombinant nucleic acid vaccine was observed using bio-fluorescence assay and muscular inoculation with hantaan viral S antigen gene fragment nucleic acid vaccine in mice to further in- vestigate the application prospect of the hantaan viral nucleic acid vaccine.Amplify and purify the constructed recom- binant plasmid pEGFP/S containing hantaan viral S antigen gone fragment and GFP(green fluorescence protein) gene,immunize the mice on shin fore-muscle and observe the pEGFP/S expression distribution on mice.Three days after the immunization GFP can be detected in liver,kidney,spleen,and muscle of the tested mice,therefore,the recombinant plasmid pEGFP/S could be expressed in many tissues and organs of the tested mice,and thus laid the foundation for further research on the nucleo-protein of hantaan virus and effective nucleic acid vaccine.