16型人乳头瘤病毒衣壳蛋白在真核细胞中的表达

为了构建HPV16型晚期蛋白重组杆状病毒,并使其在昆虫细胞中获得高效表达.首先构建2株重组杆状病毒转移质粒,分别携带人乳头瘤病毒晚期基因L1及L1和L2,再用线性化的杆状病毒DNA与该重组杆状病毒转移质粒共转染sf9昆虫细胞进行同源重组,获得2株重组杆状病毒.经鉴定该重组病毒中有目的基因存在且可表达所编码的L1或L2晚期蛋白.结果表明HPV16型晚期蛋白在昆虫细胞中获得成功表达,为HPV16型预防性基因工程亚单位疫苗的研制和诊断试剂的研究开发奠定了基础.

EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE 16 CAPSID PROTEIN IN EUKARYOTIC CELL

In order to construct a recombinant baculovirus of human papillomavirus type 16 protein at advanced stage and to express highly in insect cell, two recombinant baculovirus transfer plasmids containing human papillomavirus advanced stage genes of Li and L 1 with L 2 respectively were first constructed. Two recombinant baculovirus strains were then obtained by co transfection of linearized baculovirus DNA and the recombinant baculovirus transfer plasmids into insect cell sf9 to carry out homogenetic recombination. After appraisal the recombinant viruses did have the target genes and could express the coded advanced stage protein L 1 and L 2. The results showed that the advance stage protein of HPV16 was successfully expressed in insect cell. These have laid a foundation for the preparation of preventive genetic engineering subunit vaccine of HPV16 and the study of its diagnosis reagents.