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Characterization of Salmonella typhi OmpC and OmpF porins engineered with HIV‐gp41 epitope on the surface loops
Authors:Madhuranayaki Thulasingam  Subha Damodharan  Gopal Madhana Vigneshwari  Eswari P J Pandaranayaka  Luke Elizabeth Hanna  Ramakrishnan Usha  Sankaran Krishnaswamy
Institution:1. School of Biotechnology, Madurai Kamaraj University, Madurai, India;2. National Institute for Research in Tuberculosis, Indian Council of Medical Research, Chennai, India;3. Institute of Mathematical Sciences, Chennai, India
Abstract:Porins form trimers in the outer membrane and help transport nutrients and waste products across the bacterial cell membrane. Porin loops are suitable candidates as display systems due to their high immunogenicity and presentation at the bacterial cell surface. In this study, Salmonella typhi porins (OmpC and OmpF) engineered with the Kennedy peptide from gp41 of HIV were characterised. The chimeric OmpC carrying the Kennedy peptide in loop7 did not trimerise, whereas the chimeric OmpF with the epitope in loop5 formed trimers and also was recognised by the antibodies in the HIV patient serum. The results suggest that chimeric S. typhi OmpF may be taken further as a potential candidate to develop as an epitope display system. Proteins 2017; 85:657–664. © 2016 Wiley Periodicals, Inc.
Keywords:display system  Kennedy peptide  membrane protein  chimeric protein  trimer
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