Codon bias of the gene for chloroplast glycerol-3-phosphate acyltransferase in Camellia sinensis (L.) O. Kuntze |
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| Institution: | 1. Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy;2. Leibniz-Institut für Festkörper-und Werkstoffforschung (IFW) Dresden, P.O.Box 270116, 01171 Dresden, Germany;1. Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam;2. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam;1. Departamento de Química Inorgánica y Orgánica, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071, Jaén, Spain;2. Departamento de Química Inorgánica, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain |
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| Abstract: | The analysis on codon usage bias of GPAT gene of Camellia sinensis (L.) O. Kuntze may provide a basis for understanding the evolution relationship of C. sinensis and for selecting appropriate host expression systems to improve the expression of target genes. In the present study, the coding sequence of CsGPAT was analyzed with CodonW, CHIPS and CUSP programs, and compared with the genome of C. sinensis and GPAT genes of other 9 plant species. Our results showed that the cluster tree based on CDs could reveal the evolutional relations among the 10 plant species, whereas the cluster tree based on relative synonymous codon usage (RSCU) could not. There were 31 codons showing distinct usage differences between CsGPAT and genome of Escherichia coli, 21 between CsGPAT and yeast, but 13 between CsGPAT and Arabidopsis thaliana. But there were slightly fewer differences in codon usage between CsGPAT and A. thaliana. Therefore, the A. thaliana expression system may be more suitable for the expression of CsGPAT. These results may improve our understanding of the codon usage bias and functional studies of CsGPAT. |
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| Keywords: | Codon usage bias |
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