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Molecular characterization of Lathyrus species using chloroplast DNA trnH-psbA
Institution:1. Departamento de Farmácia, Universidade Federal de Juiz de Fora (UFJF), Campus Governador Valadares, Av. Dr. Raimundo Monteiro de Rezende, 330, Centro, 35010-177 Governador Valadares, MG, Brazil;2. Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais (UFMG), 31270-901 Belo Horizonte, MG, Brazil;3. Centro Universitário do Leste de Minas (UNILESTE), 35170-056 Coronel Fabriciano, MG, Brazil;4. LUT Chemistry, Faculty of Technology, Lappeenranta University of Technology (LUT), FI-53851 Lappeenranta, Finland;5. Departamento de Química, Centro Politécnico, Universidade Federal do Paraná (UFPR), 81531-970 Curitiba, PR, Brazil;6. Instituto Nacional de Ciência e Tecnologia de Energia e Ambiente, Instituto de Química, Universidade Federal da Bahia (UFBA), 40170-290 Salvador, BA, Brazil;1. Center of Research Excellence in Corrosion, King Fahd University of Petroleum and Minerals, Dhahran 31261, Kingdom of Saudi Arabia;2. Energy Materials Laboratory, WCU Program Department of Energy Engineering, Hanyang University, Seoul 133-791, South Korea;3. Graduate School of Biomedical Science and Engineering/College of Medicine, Hanyang University, Sungdong-gu, Seoul, South Korea;4. Department of Chemistry, Anna University, Chennai, India;1. National Institute of Biological Resources, Incheon 404-708, Republic of Korea;2. Department of Biological Sciences, Daegu University, Gyeongsan 712-714, Republic of Korea
Abstract:Lathyrus L. is an important genus contributing in human food, animal feed and fodder. The genetic variation is studied among and within six species sampled over a large geographical area: Lathyrus cicera, Lathyrus sativus, Lathyrus sylvestris, Lathyrus tuberosus, Lathyrus ochrus and Lathyrus aphaca. The phylogenetic relationship among these species was assessed using sequences of chloroplast DNA trnH-psbA (intergenic spacer). The highly polymorphic spacer' length was 330 bp. The phylogenetic analyses using Maximum Parsimony and Genetic Distances, agreed with the universal taxonomy of Kupicha. L. sativus and L. cicera could be considered as sister species, sharing a common ancestor.
Keywords:cpDNA  Phylogenetic relationships  Polymorphism  AFLP"}  {"#name":"keyword"  "$":{"id":"kwrd0040"}  "$$":[{"#name":"text"  "_":"Amplified Fragment Length Polymorphism  ISSR"}  {"#name":"keyword"  "$":{"id":"kwrd0050"}  "$$":[{"#name":"text"  "_":"Inter Simple Sequences Repeat  PCR"}  {"#name":"keyword"  "$":{"id":"kwrd0060"}  "$$":[{"#name":"text"  "_":"Polymerase Chain Reaction  RAPD"}  {"#name":"keyword"  "$":{"id":"kwrd0070"}  "$$":[{"#name":"text"  "_":"Random Amplified Polymorphic DNA  RFLP"}  {"#name":"keyword"  "$":{"id":"kwrd0080"}  "$$":[{"#name":"text"  "_":"Restriction Fragment Length Polymorphism
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