Genetic relationships among Mexican white pines (Pinus, Pinaceae) based on RAPD markers |
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Authors: | Patricia Castro-Flix Jorge A Prez de la Rosa Georgina Vargas Amado Salvador Velsquez Magaa Anne Santerre F Lpez-Dellamary Toral Alma R Villalobos-Armbula |
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Institution: | aDepartamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Colonia Sante Fe, 45160 Zapopan, Jalisco, Mexico;bDepartamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, 45110, Zapopan, Jalisco, Mexico;cDepartamento de Madera Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingeniería. Universidad de Guadalajara, Guadalajara, Mexico |
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Abstract: | Genetic relationships among Mexican white pines have not been completely resolved by DNA sequencing analyses. The use of random amplified polymorphic DNA (RAPD) markers for the study of interspecific relationships has been questioned because of the possible lack of homology of co-migrating bands between species. However, several RAPD based studies on pines have provided sufficient information to discriminate between closely related taxa. Genetic relationships among four species of Mexican white pines (Pinus ayacahuite, Pinus strobiformis, Pinus lambertiana and Pinus chiapensis) were estimated based on RAPD markers. Sixty-nine primers generated 247 bands in pooled DNA samples from ten populations. In addition, four selected primers generated 27 bands in 176 individual DNA samples. Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendrograms based on Jaccard similarity indices were constructed. The results suggest that the closest pine species analyzed were P. ayacahuite and P. strobiformis, followed by P. lambertiana. The most genetically distant species was P. chiapensis. Cluster analyses did not support P. strobiformis as a distinct species from P. ayacahuite. |
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Keywords: | Pinus strobiformis– P ayacahuite complex Pinus lambertiana Pinus chiapensis Random amplified polymorphic DNA Genetic relationships Genetic diversity |
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