Isolation and characterization of microsatellite loci for the analysis of genetic diversity in Whitmania pigra |
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| Institution: | 1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, PR China;2. School of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng 224051, PR China;3. Department of Animal Nutrition and Feed Sciences, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China;1. Chemical Physics of Materials and Catalysis, Université libre de Bruxelles, CP243 Brussels, Belgium;2. Institut für Physikalische Chemie und Elektrochemie, Leibniz Universität Hannover, Hannover, Germany;3. Center for Nonlinear Phenomena and Complex Systems (CENOLI), Université libre de Bruxelles, CP231 Brussels, Belgium;1. State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150080, China;2. The Fourth Hospital of Harbin Medical University, Harbin 150001, China;3. Sino-Russia Joint Lab for High Energy Beam, Shenyang Ligong University, Shenyang 110159, China;4. School of Automobile and Traffic Engineering, Heilongjiang Institute of Technology, Harbin 150050, China;5. Shanghai Key Laboratory of Advanced High-temperature Materials and Precision Forming, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, China;6. The State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, China;1. Mechanical & Aerospace Engineering, Oklahoma State University, Tulsa, 74106, OK, United States;2. Center for Nanoscale Materials, Argonne National Laboratory, Argonne, 60439, IL, United States;1. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;2. College of Pharmacy, Yanbian University, Yanji 133002, PR China;3. College of Pharmacy, Duksung Women''s University, 33, Samyang-ro 144-gil, Dobong-gu, Seoul 132-714, Republic of Korea;4. College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea;1. School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland;2. Tyndall National Institute, University College Cork, Lee Maltings, Cork, Ireland;3. National Institute of Standards and Technology, Gaithesburg, MD 20899, USA;4. U.S. Army Research Laboratory, Adelphi, MD 20783, USA;1. Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China;2. School of Materials and Mechanical & Electrical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China;3. Department of Chemistry, Nanchang University, Nanchang 330013, PR China |
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| Abstract: | The objective of this study was to isolate microsatellite loci to analyze the genetic diversity of Whitmania pigra. Four new microsatellite markers of W. pigra were developed from an enriched library and ten from a modified SAMPL assay. A total of 127 alleles were detected, with an average of 9.1 alleles per locus. The expected heterozygosity (He) of each microsatellite locus varied from 0.451 to 0.857, with an average of 0.688. The polymorphism information content (PIC) of each microsatellite locus ranged from 0.361 to 0.838, with an average of 0.640. Analysis of molecular variance showed that the main variation component existed within the populations (81.64%) rather than among the populations (18.36%). Phylogenetic tree for 15 populations of Hirudo using the NJ method by MEGA 5.1 software were divided into two major clusters. These microsatellite markers will contribute to research on the individual identification, genetic diversity, population structure, genome mapping and conservation biology of Hirudo. |
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| Keywords: | Isolation Microsatellite Genetic diversity |
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