ISSR analysis of genetic diversity of the Qinghai-Tibet Plateau endemic Rhodiola chrysanthemifolia (Crassulaceae) |
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Authors: | Tao Xia Shilong Chen Shengyun Chen Defang Zhang Dejun Zhang Qingbo Gao Xuejun Ge |
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Institution: | 1. Laboratory of Qinghai-Tibet Biological Evolution and Adaptation, Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001, PR China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China;3. South China Institute of Botany, The Chinese Academy of Sciences, Guangzhou 510650, PR China |
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Abstract: | Inter-simple sequence repeat markers (ISSR) were used to estimate genetic diversity within and among 10 populations of Rhodiola chrysanthemifolia along Nianqingtangula Mountains and Brahmaputra, a species endemic to the Qinghai-Tibet Plateau and an endangered medicinal plant. Of the 100 primers screened, 13 produced highly polymorphic DNA fragments. Using these primers, 116 discernible DNA fragments were generated of which 104 (89.7%) were polymorphic, indicating substantial genetic diversity at the species level. Genetic diversity measured by the percentage of polymorphic bands (PPB) at the population level ranged from 21.97% to 48.8%. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (77.3%), but no regional differentiation was discernible. Variance within populations was only 22.7%. The main factor responsible for this high level of differentiation among populations is probably the historical geographical and genetic isolation of populations in a harsh mountainous environment. Concerning the management of R. chrysanthemifolia, the high genetic differentiation of populations indicates the necessity of conserving the maximum possible number of populations. |
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Keywords: | Rhodiola chrysanthemifolia ISSRs Genetic diversity Qinghai-Tibet Plateau |
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