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Fifteen new polymorphic microsatellite loci for the meadow brown butterfly,Maniola jurtina
Institution:1. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, PR China;2. College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China
Abstract:We characterized fifteen microsatellite markers for the butterfly Maniola jurtina. For the six studied populations (96 samples) the total number of alleles per locus ranged from 3 to 55 and mean overall expected heterozygosity across all loci was 0.74. In spite of a high frequency of null alleles detected in part of the loci, a recurrent phenomenon in Lepidopteron, the estimation of pairwise FST seems rather insensitive to the presence of these null alleles as shown by the high correlation between FST calculated after correction for the presence of null alleles and non-corrected FST, indicating that the loci may be usable in population genetics, more specifically for the study of populations genetics structure.
Keywords:Microsatellite  Maniola jurtina  454 method  Population genetics  France
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