Sequence diversity of the MHC Ⅱ DRB gene in Chinese tree shrews (Tupaia belangeri chinensis) |
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| Institution: | 1. Department of Cardiology, Chinese PLA General Hospital, Beijing, China;2. Department of Health Statistics, Fourth Military Medical University, Chinese PLA Police Force Center for Disease Control and Prevention, Xi''an, Shanxi Province, China;3. Department of cardiology, Yishui Center hospital of Shandong Province, Linyi, China;4. Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China;5. Division of Human Genetics, Cincinnati Children''s Hospital Medical Center, Cincinnati, OH, USA;6. Division of Pathology, Cincinnati Children''s Hospital Medical Center, Cincinnati, OH, USA;1. Department of Parasitology, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan;2. Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China;3. Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan;4. Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand;1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;2. School of Animal Science & Technology, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | The major histocompatibility complex (MHC) is a cluster of genes involved in vertebrate immune response regulation. MHC class I and II cell surface proteins are crucial for discrimination of self versus non-self by the adaptive immune system. Due to their special phylogenetic position within the Euarchontoglires and as a relative of primates, tree shrews have been proposed as an alternative experimental animal model for biomedical studies. However, information about the genetic structure of the tree shrew populations is largely unknown. In this study, we characterized diversity in exon 2 of the MHC II DRB gene isolated from Chinese tree shrews (Tupaia belangeri chinensis). We identified 12 different DRB exon 2 alleles from 15 Chinese tree shrews, 1 to 4 alleles were observed per individual with high levels of sequence divergence between alleles. There were more non-synonymous than synonymous substitutions in the functionally important antigen-binding site (dN/dS = 2.7952, P < 0.01), indicating that the DRB exon 2 in Chinese tree shrews has been influenced by positive selection. |
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| Keywords: | Major histocompatibility complex Tupaia belangeri chinensis DRB exon 2 Diversity Antigen binding region Positive selection |
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