SSR markers for a critically endangered species Euryodendron excelsum and a distantly related species Ternstroemia gymnanthera (Ternstroemiaceae) |
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| Institution: | 1. CEPROCOR, Álvarez de Arenales 230, B° Juniors, 5000 Córdoba, Argentina;2. Facultad de Matemática, Astronomía y Física (FAMAF), Ciudad Universitaria, M. Allende y H. de la Torre, Córdoba, Argentina;3. CONICET, Rivadavia 1917, 1033 Buenos Aires, Argentina;4. LNLS, Laboratorio Nacional de Luz Sincrotron, Rua Giuseppe Máximo Scolfaro, 10000, Guará, Campinas, SP 13083-100, Brazil;1. State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, China;2. Center for Excellence in Life and Paleoenvironment, Chinese Academy of Sciences, Nanjing 210008, China;3. School of Earth Sciences & Key Laboratory of Mineral Resources in Western China (Gansu Province), Lanzhou University, Lanzhou 730000, China |
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| Abstract: | Euryodendron excelsum is a critically endangered tertiary relict plant endemic to China. It has only one population remaining in Ba Jia Zhen of Yangchun, Guangdong. In this study, we discovered 25 microsatellite markers from E. excelsumusing a Fast Isolation by Amplified Fragment Length Polymorphism of Sequences COntaining Repeats (FIASCO) protocol. Thirteen loci demonstrated polymorphisms, with the number of alleles per locus ranging from 2 to 13. Values for observed and expected heterozygosity ranged from 0.176 to 1.000 and from 0.261 to 0.889, respectively. Three loci (ZXM-17, ZXM-54, and ZXM-92) were found to significantly deviate from Hardy-Weinberg equilibrium. In addition, five of 13 loci could be successfully cross-amplified in Ternstroemia gymnanthera. These microsatellite loci may help to further survey the adaptive evolution and genetic variation of E. excelsum for guiding its conservation. |
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| Keywords: | Microsatellite markers Genetic analysis Cross-amplification |
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