Chromosomal locations of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis |
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| Authors: | Kun-Sheng Wu Jean-Christophe Glaszmann Gurdev S Khush |
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| Institution: | (1) Present address: Department of Agronomy, South China Agricultural University, Guangzhou, China;(2) Institut de Recherches Agronomiques Tropicales et des Cultures Vivrières, Centre de Cooperation Internationale en Recherche Agronomique pour le Developpment, B.P. 5035, 34032 Montpellier, Cedex, France;(3) International Rice Research Institute, P. O. Box 933, Manila, Philippines |
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| Abstract: | Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F1 trisomics. This served as the primary source of evidence for chromosome locations ofEst-5, Icd-1, Acp-1, andPgd-1. The locations ofAmp-1, Amp-2, Amp-4, Pox-5, Got-1, andCat-1 were further confirmed from segregation data in BC1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but agreed with the expected trisomic ratios. Triallelic heterozygotes were recovered forAmp-1 andAmp-2. On the basis of these dataGot-1, Est-5, andIcd-1 were located to chromosome 1,Amp-1 to chromosome 2,Cat-1 andPox-5 to chromosome 3,Acp-1 to chromosome 6,Amp-2 andAmp-4 to chromosome 8, andPgd-1 to chromosome 11. BecauseAcp-2 andPox-2 are known to be linked withAcp-1, they must also be on chromosome 6. The gene order and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are presented.The senior author wishes to acknowledge the financial support from the Chinese government. |
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| Keywords: | isozymes trisomics linkage relations chromosome maps |
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