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Serial electrophoretic transfers: A technique for the identification of numerous enzymes from single polyacrylamide gels |
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| Authors: | Tracy McLellan John A M Ramshaw |
| Institution: | (1) Museum of Comparative Zoology, Harvard University, 02138 Cambridge, Massachusetts;(2) Present address: Division of Medical Genetics, Department of Pediatrics, University of Utah Medical Center, 84132 Salt Lake City, Utah;(3) Present address: Division of Protein Chemistry, C.S.I.R.O., Parkville, Victoria, Australia |
| Abstract: | We describe an electrophoretic method for the transfer of macromolecules from polyacrylamide slab gels to ion-exchange paper without loss of clarity or resolution. A series of partial transfers provides numerous copies of a single gel separation, and these copies may be assayed independently with enzyme specific stains. This method therefore combines the advantages of polyacrylamide gel electrophoresis for detecting enzyme variation with an efficient method for examining numerous enzymes from a single gel separation.This work was supported by NIH Grants GM 24849 and GM 27119 to R. C. Lewontin. |
| Keywords: | polyacrylamide electrophoresis allelic variation multiple enzyme stains electrophoretic transfer |
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