茶树品种、叶片生育期和茶叶化学成份对内生真菌的影响

通过茶树品种、叶片龄期及叶片化学成份对茶树内生真菌分布影响的研究,发现:(1)在供试的4个茶树品种中,黄旦的内生真菌分离频率达100%,而福云六号只有7.5%,表明茶树品种不同或遗传背景不同,其叶片中内生真菌的发生存在明显差异。(2)随着茶树叶片的成熟,叶片中内生真菌的种类与数量逐渐增多,与叶片龄期呈正相关,叶片龄期影响茶树内生真菌的种群结构。(3)不同龄期叶片水浸提液对Colletotrichum、Pestalotiopsis、Guignardia三种茶树上的主要内生真菌的生长产生不同的影响;在PDA培养基中添加15%的茶多酚,对此3种内生真菌均有一定的抑制作用,但抑制程度有所不同,表明茶树叶片的化学成份是影响内生真菌分布的重要因素之一。

Effect of Tea Variety, Leaf Age and Chemical Component on Endophytic Fungi

After the study of the effect of tea variety,leaf age and chemical component on endophytic fungi,it was found that: 1.Among the four tested tea varieties,the isolation ration of endophytic fungi from variety Huangdan was 100%, while that of variety Fuyun-6 only 7.5%,it shows that varieties or genetic background of Camellia sinensis significantly affect the incidence of endophytic fungi in tea leaves.2.During the tea leaves become matured or aged,the endophytic fungal species and amount increase gradually,and they have a positive relationship,the leaf age affects the population constituent of endophytic fungi.3.The water extraction of tea leaves differently affected the growth of Colletotrichum,Pestalotiopsis and Guignardia species,the three important endophytic fungi.Addition of 15% tea polyphenol in PDA resulted in different level of inhibition to these fungal growths.It also implies that the chemical component is one of the factors affecting incidence and distribution of endophytic fungi in tea leaves.